A Potential Role of CD82/KAI1 during Uterine Decidualization in Mice

作者全名："Li, Qijun; Song, Mengyao; Cao, Ke; Zhang, Qian"

作者地址："[Li, Qijun; Song, Mengyao; Cao, Ke; Zhang, Qian] Chongqing Med Univ, Lab Anim Ctr, Chongqing 400016, Peoples R China; [Zhang, Qian] Chongqing Engn Res Ctr Rodent Lab Anim, Chongqing, Peoples R China"

通信作者："Zhang, Q (通讯作者)，Chongqing Med Univ, Lab Anim Ctr, Chongqing 400016, Peoples R China.; Zhang, Q (通讯作者)，Chongqing Engn Res Ctr Rodent Lab Anim, Chongqing, Peoples R China."

来源：CURRENT ISSUES IN MOLECULAR BIOLOGY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001191783700001

JCR分区：Q4

影响因子：3.1

年份：2024

卷号：46

期号：3

开始页：1799

结束页：1809

文献类型：Article

关键词：CD82/KAI1; decidualization; stromal cells; uterus; mouse

摘要："The tumor metastasis suppressor gene CD82/KAI1 has been demonstrated to impact human trophoblast invasion and migration. Communication between trophoblasts and decidual stromal cells plays a crucial role in controlling the normal invasiveness of trophoblasts. However, whether CD82/KAI1 is involved in decidualization and what role it plays remain unclear. CD82/KAI1 demonstrates specific spatiotemporal expression patterns in stromal cells undergoing decidualization during pregnancy. This is observed in both naturally pregnant females post-implantation and pseudopregnant mice undergoing induced decidualization, as detected through in situ hybridization and immunofluorescence. CD82/KAI1 expression showed a significant time-dependent increase in cultured stromal cells after 24 and 48 h of progesterone (P4) and estrogen (E2) treatment. This was accompanied by a notable upregulation of decidualization markers, including cyclin D3 and PR. After transducing stromal cells with the adenovirus-overexpressing CD82/KAI1 for 48 h, the expression of cyclin D3 protein increased. Meanwhile, there was an attenuated expression of CD82/KAI1 due to an adenovirus siRNA knockdown, whereas cyclin D3 and PR expressions were not affected. Our findings suggest a potential role of CD82/KAI1 in regulating the process of decidualization, providing insights into stromal cell differentiation."

基金机构：Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJQN202100411]; Young Elite Scientists Sponsorship Program [2016QNRC001]

基金资助正文：This study was supported by the Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJQN202100411 to Q.Z.) Young Elite Scientists Sponsorship Program (2016QNRC001 to Q.Z.).