"Effects of silencing hsa_circ_0015326 on proliferation, migration, invasion, and apoptosis of epithelial ovarian cancer cells"

作者全名："Zhang, Cui-Ying; Liu, Wei; Wang, Jia; Zhang, Wen-Wen; Huang, Jing-Lin; Huang, Xi-Yue; Zhang, Ying-Feng; Li, Chang-Jiang; Wang, Ting-Ting; Mao, Yan-Hua; Wang, Wen-Min; Sun, Cong-Cong"

作者地址："[Zhang, Cui-Ying] Chongqing Med Univ, Dept Gynecol, Yongchuan Hosp, Chongqing, Peoples R China; [Liu, Wei] Chongqing Med Univ, Dept Orthoped, Yongchuan Hosp, Chongqing, Peoples R China; [Wang, Jia; Zhang, Wen-Wen; Huang, Jing-Lin; Huang, Xi-Yue; Zhang, Ying-Feng; Li, Chang-Jiang; Wang, Ting-Ting; Mao, Yan-Hua; Sun, Cong-Cong] Chongqing Med Univ, Dept Gynecol & Obstet, Univ Town Hosp, Chongqing, Peoples R China; [Wang, Wen-Min] Laisu Town Hlth Ctr Yongchuan, Dept Gynecol & Obstet, Chongqing, Peoples R China; [Sun, Cong-Cong] Chongqing Med Univ, Town Hosp Hosp, Dept Gynecol & Obstet, 55 Univ Town Middle Rd, Chongqing 401331, Peoples R China"

通信作者："Sun, CC (通讯作者)，Chongqing Med Univ, Town Hosp Hosp, Dept Gynecol & Obstet, 55 Univ Town Middle Rd, Chongqing 401331, Peoples R China."

来源：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001194762900001

JCR分区：Q2

影响因子：3.6

年份：2024

卷号：38

期号：4

开始页：　

结束页：　

文献类型：Article

关键词：apoptosis; hsa_circ_0015326; invasion; migration; ovarian cancer

摘要："Although the treatment of ovarian cancer has made great progress, there are still many patients who are not timely detected and given targeted therapy due to unknown pathogenesis. Recent studies have found that hsa_circ_0015326 is upregulated in ovarian cancer and is involved in the proliferation, invasion, and migration of ovarian cancer cells. However, whether hsa_circ_0015326 can be used as a new target of ovarian cancer needs further investigation. Therefore, the effect of hsa_circ_0015326 on epithelial ovarian cancer was investigated in this study. At first, si-hsa_circ_0015326 lentivirus was transfected into epithelial ovarian cancer cells. Then real-time fluorescence quantitative PCR (qRT-PCR) was used to detect hsa_circ_0015326 level. The proliferation of ovarian cancer cells was detected by CCK-8 assay. The horizontal and vertical migration abilities of the cells were detected by wound-healing assay and Transwell assay, respectively. Transwell assay was also used to determine the invasion rate. As for the apoptosis rate, it was assessed by flow cytometry. As a result, the expression level of hsa_circ_0015326 in A2780 and SKOV3 was found to be higher than that in IOSE-80. However, after transfecting si-hsa_circ_0015326 and si-NC into the cells, the proliferation, migration, and invasion abilities of A2780 and SKOV3 cells in the si-hsa_circ_0015326 group were significantly reduced in comparison to those in the si-NC and mock groups, while their apoptosis rates were elevated. Collectively, silencing hsa_circ_0015326 bears the capability of inhibiting the proliferation, migration, and invasion of ovarian cancer cells while increasing apoptosis rate. It can be concluded that hsa_circ_0015326 promotes the malignant biological activities of epithelial ovarian cancer cells. si-hsa_circ_0015326 lentivirus was transfected into epithelial ovarian cancer cells. Then real-time fluorescence quantitative PCR (qRT-PCR) was used to detect hsa_circ_0015326 level. The proliferation of ovarian cancer cells was detected by CCK-8 assay. The horizontal and vertical migration abilities of the cells were detected by wound-healing assay and Transwell assay, respectively. Transwell assay was also used to determine the invasion rate. As for the apoptosis rate, it was assessed by flow cytometry. As a result, Hsa_circ_0015326 is highly expressed in ovarian cancer cells. And silencing hsa_circ_0015326 inhibits the proliferation, migration, and invasion abilities of the cells. Therefore, hsa_circ_0015326 is speculated to be an oncogene and plays a significant role in the genesis and development of ovarian cancer, which might become a new biomarker for the early diagnosis of patients with ovarian cancer. image"

基金机构：Science and Technology Research Program of Chongqing Municipal Education Commission; Yongchuan Natural Science Foundation [2020nb0228]; Chongqing Graduate Research Innovation Project [CYS22386]; Chongqing Talent Program [cstc2021ycjh-bgzxm0014]; [KJQN202000434]

基金资助正文："This study was supported by the Science and Technology Research Program of Chongqing Municipal Education Commission (No. KJQN202000434), Yongchuan Natural Science Foundation (No. 2020nb0228), Chongqing Graduate Research Innovation Project (No. CYS21249), Chongqing Graduate Research Innovation Project (No. CYS22386), Chongqing Talent Program (cstc2021ycjh-bgzxm0014)."