Role of oncogenic long noncoding RNA KCNQ1OT1 in colon cancer

作者全名："Liu, Gang; Shi, Lei; Wang, Bin; Wu, Zehui; Zhao, Haiyuan; Zhao, Tianyu; Shi, Lianghui"

作者地址："[Liu, Gang; Shi, Lei; Wang, Bin; Wu, Zehui; Zhao, Haiyuan; Shi, Lianghui] Wannan Med Coll, Affiliated Hosp 1, Dept Gastrointestinal Surg, Wuhu 241001, Peoples R China; [Zhao, Tianyu] Chongqing Med Univ, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China"

通信作者："Shi, LH (通讯作者)，Wannan Med Coll, Affiliated Hosp 1, Dept Gastrointestinal Surg, Wuhu 241001, Peoples R China."

来源：ONCOLOGY RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001196684600002

JCR分区：Q2

影响因子：3.1

年份：2024

卷号：32

期号：3

开始页：585

结束页：596

文献类型：Article

关键词：lncRNA KCNQ1OT1; Colon cancer; HCT116 cells; Tumorigenesis

摘要："The role of lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in colon cancer involves various tumorigenic processes and has been studied widely. However, the mechanism by which it promotes colon cancer remains unclear. Retroviral vector pSEB61 was retrofitted in established HCT116-siKCN and SW480-siKCN cells to silence KCNQ1OT1. Cellular proliferation was measured using CCK8 assay, and flow cytometry (FCM) detected cell cycle changes. RNA sequencing (RNA-Seq) analysis showed differentially expressed genes (DEGs). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out to analyze enriched functions and signaling pathways. RT-qPCR, immunofluorescence, and western blotting were carried out to validate downstream gene expressions. The effects of tumorigenesis were evaluated in BALB/c nude mice by tumor xenografts. Our data revealed that the silencing of KCNQ1OT1 in HCT116 and SW480 cells slowed cell growth and decreased the number of cells in the G2/M phase. RNA-Seq analysis showed the data of DEGs enriched in various GO and KEGG pathways such as DNA replication and cell cycle. RT-qPCR, immunofluorescence, and western blotting confirmed downstream CCNE2 and PCNA gene expressions. HCT116-siKCN cells significantly suppressed tumorigenesis in BALB/c nude mice. Our study suggests that lncRNA KCNQ1OT1 may provide a promising therapeutic strategy for colon cancer."

基金机构：Scientific Research Project of Anhui Provincial Health Commission [AHWJ2021b109]; Scientific and Technological Research Program of Chongqing Municipal Education Commission [KJZD-K201900402]; Special Fund for Wannan Medical College Scholar Project [WK2021F07]; Educational Commission of Anhui Province of China [2022AH051241]

基金资助正文："This work was financially supported by the Scientific Research Project of Anhui Provincial Health Commission in 2021 (# AHWJ2021b109 to LS), Scientific and Technological Research Program of Chongqing Municipal Education Commission (# KJZD-K201900402 to TZ), Special Fund for Wannan Medical College Scholar Project (# WK2021F07), and Educational Commission of Anhui Province of China (2022AH051241)."