Low expression of fatty acid oxidation related gene ACADM indicates poor prognosis of renal clear cell carcinoma and is related to tumor immune infiltration

作者全名："Qiu, Jiechuan; Yang, Tianmin; Sun, Yanning; Sun, Kai; Xu, Yingkun; Xia, Qinghua"

作者地址："[Qiu, Jiechuan; Yang, Tianmin; Xia, Qinghua] Shandong First Med Univ, Dept Urol, Shandong Prov Hosp, Jinan 250021, Peoples R China; [Sun, Yanning; Sun, Kai] Shandong Univ, Shandong Prov Hosp, Cheeloo Coll Med, Dept Urol, Jinan 250021, Peoples R China; [Xu, Yingkun] Chongqing Med Univ, Affiliated Hosp 1, Dept Breast & Thyroid Surg, Chongqing 400042, Peoples R China"

通信作者："Xia, QH (通讯作者)，Shandong First Med Univ, Dept Urol, Shandong Prov Hosp, Jinan 250021, Peoples R China."

来源：ONCOLOGY RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001196918400002

JCR分区：Q2

影响因子：3.1

年份：2024

卷号：32

期号：3

开始页：545

结束页：561

文献类型：Article

关键词：Kidney renal clear cell carcinoma; Acyl-CoA dehydrogenase medium chain; Immune infiltration; Fatty acid oxidation; Prognosis

摘要："This research aims to identify the key fatty acid beta-oxidation (FAO) genes that are altered in kidney renal clear cell carcinoma (KIRC) and to analyze the role of these genes in KIRC. The Gene Expression Omnibus (GEO) and FAO datasets were used to identify these key genes. Wilcoxon rank sum test was used to assess the levels of acylCoA dehydrogenase medium chain (ACADM) between KIRC and non-cancer samples. The logistic regression and Wilcoxon rank sum test were used to explore the association between ACADM and clinical features. The diagnostic performance of ACADM for KIRC was assessed using a diagnostic receiver operating characteristic (ROC) curve. The co-expressed genes of ACADM were identified in LinkedOmics database, and their function and pathway enrichment were analyzed. The correlation between ACADM expression level and immune infiltration was analyzed by Gene Set Variation Analysis (GSVA) method. Additionally, the proliferation, migration, and invasion abilities of KIRC cells were assessed after overexpressing ACADM. Following differential analysis and intersection, we identified six hub genes, including ACADM. We found that the expression level of ACADM was decreased in KIRC tissues and had a better diagnostic effect (AUC = 0.916). Survival analysis suggested that patients with decreased ACADM expression had a worse prognosis. According to correlation analysis, a variety of clinical features were associated with the expression level of ACADM. By analyzing the infiltration level of immune cells, we found that ACADM may be related to the enrichment of immune cells. Finally, ACADM overexpression inhibited proliferation, migration, and invasion of KIRC cells. In conclusion, our findings suggest that reduced ACADM expression in KIRC patients is indicative of poor prognosis. These results imply that ACADM may be a diagnostic and prognostic marker for individuals with KIRC, offering a reference for clinicians in diagnosis and treatment."

基金机构："National Natural Science Foundation of China [82072816, 81672553]; Natural Science Foundation of Shandong Province [ZR2021LZY003]"

基金资助正文：Funding Statement: This research was funded by the National Natural Science Foundation of China (Grant Nos. 82072816 and 81672553) and the Natural Science Foundation of Shandong Province (Grant No. ZR2021LZY003) .