Multi-omics data-based analysis characterizes molecular alterations of the vesicle genes in human colorectal cancer

作者全名："Wang, Xi; Wu, Min-Min; Zhang, Wei; Liu, Zhen-Qiong; Xu, Meng-Qi; Zhang, Feng-Mei; He, Zhi-Qiang; Tang, Dong-E; Tang, Min; Dai, Yong"

作者地址："[Wang, Xi; Zhang, Wei; Tang, Dong-E; Dai, Yong] Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Clin Med Res Ctr, Shenzhen 518055, Guangdong, Peoples R China; [Wu, Min-Min; Zhang, Wei] Shenzhen Univ, South China Hosp, Hlth Sci Ctr, Shenzhen 518020, Guangdong, Peoples R China; [Wu, Min-Min; Liu, Zhen-Qiong; Xu, Meng-Qi; Zhang, Feng-Mei; He, Zhi-Qiang; Tang, Min] Chongqing Med Univ, Key Lab Diagnost Med, Chinese Minist Educ, Chongqing 400016, Peoples R China; [Dai, Yong] Anhui Univ Sci & Technol, Affiliated Hosp 1, Sch Med, Huainan 232001, Anhui, Peoples R China; [Dai, Yong] Peking Univ, Dept Rheumatism & Immunol, Key Lab Inflammatory & Immunol Dis, Shenzhen Hosp, Shenzhen 518036, Guangdong, Peoples R China"

通信作者："Tang, DE; Dai, Y (通讯作者)，Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Clin Med Res Ctr, Shenzhen 518055, Guangdong, Peoples R China.; Tang, M (通讯作者)，Chongqing Med Univ, Key Lab Diagnost Med, Chinese Minist Educ, Chongqing 400016, Peoples R China."

来源：AMERICAN JOURNAL OF CANCER RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001196985900002

JCR分区：Q2

影响因子：5.3

年份：2024

卷号：14

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：Human colorectal cancer; vesicle genes; multi-omics analysis; mechanisms of disease; therapeutic targets

摘要："The role of vesicular genes in the development of colorectal cancer (CRC) is crucial. Analyzing alterations in these genes at multi-omics can aid in understanding the molecular pathways behind colorectal carcinogenesis and identifying potential treatment targets. However, studies on the overall alteration of vesicular genes in CRC are still lacking. In this study, we aimed to investigate the relationship between vesicle genetic alterations and CRC progression. To achieve this, we analyzed molecular alterations in CRC vesicle genes at eight levels, including mRNA, protein, and epigenetic levels. Additionally, we examined CRC overall survival -related genes that were obtained from a public database. Our analysis of chromatin structural variants, DNA methylation, chromatin accessibility, and proteins (including phosphorylation, ubiquitination, and malonylation), along with RNA-seq data from the TCGA database, revealed multiple levels of alterations in CRC vesicle genes in the collected tissue samples. We progressively examined the alterations of vesicle genes in mRNA and protein levels in CRC and discovered the hub genes. Further investigation identified the probable essential transcription factors. This study contributes to a thorough knowledge of the connection between vesicle gene alterations at multiple levels and the development of CRC and offers a theoretical framework for the identification of novel treatment targets."

基金机构：National Natural Science Foundation of China [82003172]; Shenzhen Fund for Guangdong Provincial High level Clinical Key Specialties [SZGSP001]; Postdoctoral Science Foundation of China [2020M673065]; Natural Science Foundation of Guangdong Province [2019A1515-111138]; Science and technology plan of Shenzhen [JCYJ20180306140810282]

基金资助正文："<STRONG> </STRONG>This study was approved by the National Natural Science Foundation of China (No. 82003172) , Shenzhen Fund for Guangdong Provincial High level Clinical Key Specialties (No. SZGSP001) , the Postdoctoral Science Foundation of China (No. 2020M673065) , Natural Science Foundation of Guangdong Province (No. 2019A1515-111138) , and the science and technology plan of Shenzhen (No. JCYJ20180306140810282) ."