"SPOP attenuates the proliferation, invasion, and migration of choriocarcinoma JAR cells by promoting KIF23 degradation"

作者全名："Zhou, Chunli; Chen, Yiyu; Jiang, Hairong; Xia, Chenchen; Yuan, Xiaohan; Yu, Qiubo"

作者地址："[Yu, Qiubo] Chongqing Med Univ, Mol Med Lab, Chongqing 400016, Peoples R China; [Yu, Qiubo] Chongqing Med Univ, Dept Pathol, Chongqing 400016, Peoples R China; [Zhou, Chunli; Chen, Yiyu; Xia, Chenchen; Yuan, Xiaohan] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China; [Jiang, Hairong] Chongqing Publ Hlth Med Ctr, Dept Clin Lab, Chonging, Peoples R China"

通信作者："Yu, QB (通讯作者)，Chongqing Med Univ, Mol Med Lab, Chongqing 400016, Peoples R China.; Yu, QB (通讯作者)，Chongqing Med Univ, Dept Pathol, Chongqing 400016, Peoples R China."

来源：ONCOLOGIE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001199845100001

JCR分区：Q4

影响因子：0.9

年份：2024

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：choriocarcinoma; speckle-type POZ protein (SPOP); kinesin-like protein KIF23 (KIF23); ubiquitination-modified quantitative proteomics

摘要："Objectives: Choriocarcinoma is a highly malignant and aggressive trophoblastic tumor. In our previous study, we discovered that the speckle-type POZ protein (SPOP), which recognizes substrates of E3 ubiquitin ligase, plays a crucial role in trophoblast-derived choriocarcinoma cell lines. Therefore, we investigated the correlation between SPOP and the substrate kinesin-like protein KIF23, as well as the role of KIF23 in choriocarcinoma cells. Methods: We constructed JAR cells overexpressing SPOP using lentiviral vectors and subsequently screened the related proteins through ubiquitination-modified quantitative proteomic analysis. The relationship between KIF23 and SPOP was determined using western blotting, and CCK-8, plate cloning, flow cytometry, and Transwell assays were used to investigate the effects of KIF23 and SPOP/KIF23. Results: We identified the KIF23 protein and observed that SPOP promoted its degradation. The abundance of KIF23 increased after the addition of the protease inhibitor MG132. KIF23 was highly expressed in choriocarcinoma cells. Compared with JAR cells transfected with NC-small-interfering RNA (siRNA), the proliferation, invasion, migration, and percentage of G0/G1 cells in the KIF23-siRNA group were significantly lower, and the activation of the Akt/GSK3 beta signaling pathway was markedly attenuated. Additionally, the sh-SPOP+KIF23-siRNA group exhibited significantly inhibited JAR cell proliferation, invasion, and migration, along with clearly attenuated activation of the Akt/GSK3 beta signaling pathway. Conclusions: SPOP attenuates the proliferation, invasion, and migration of choriocarcinoma JAR cells by promoting KIF23 degradation."

基金机构：Chongqing Science and Technology Commission ProjectChongqing Medical University; Institute of Life Sciences of Chongqing Medical University

基金资助正文：All authors thank the Institute of Life Sciences of Chongqing Medical University for providing the laboratory for this study.