Gastrodin protects endothelial cells against high glucose-induced injury through up-regulation of PPAR? and alleviation of nitrative stress

作者全名："Yang, Chuang; Qiu, Hongmei; Lv, Mingqi; Yang, Junxia; Wu, Ke; Huang, Jiajun; Jiang, Qingsong"

作者地址："[Yang, Chuang; Qiu, Hongmei; Yang, Junxia; Wu, Ke; Huang, Jiajun; Jiang, Qingsong] Chongqing Med Univ, Coll Pharm, Dept Pharmacol, Chongqing Key Lab Biochem & Mol Pharmacol, Chongqing 400016, Peoples R China; [Lv, Mingqi] Chongqing Med Univ, Expt Teaching Management Ctr, Chongqing 400016, Peoples R China"

通信作者："Jiang, QS (通讯作者)，Chongqing Med Univ, Coll Pharm, Dept Pharmacol, Chongqing Key Lab Biochem & Mol Pharmacol, Chongqing 400016, Peoples R China."

来源：MICROVASCULAR RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000965258100001

JCR分区：Q2

影响因子：2.9

年份：2023

卷号：148

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Gastrodin; PPAR ?; Nitrative stress; High glucose; Vascular endothelial cells

摘要："In diabetes mellitus (DM), high glucose can result in endothelial cell injury, and then lead to diabetic vascular complications. Gastrodin, as the mainly components of Chinese traditional herb Tianma (Gastrodia elata Bl.), has been widely used for cardiovascular diseases. However, the known of the effect of gastrodin on endothelial cell injury is still limited. In this study, we aimed to investigate the effect and possible mechanism of gastrodin on high glucose-injured human umbilical vein endothelial cells (HUVEC). High glucose (30 mmol/L) treatment caused HUVEC injury. After gastrodin (0.1, 1, 10 mu mol/L) treatment, compared with the high glucose group, the cell proliferation ability increased in a dose-dependent manner. Meanwhile, gastrodin (10 mu mol/L) up-regulated the mRNA and protein expressions of PPAR beta and eNOS, decreased the expressions of iNOS, also reduced the protein expression of 3-nitrotyrosine, and lowed the level of ONOO-, increased NO content. Both the PPAR beta antagonist GSK0660 (1 mu mol/L) and the eNOS inhibitor L-NAME (10 mu mol/L) were able to block the above effects of gastrodin. In conclusion, gastrodin protectes vascular endothelial cells from high glucose injury, which may be, at least partly, mediated by up-regulating the expression of PPAR beta and negatively regulating nitrative stress."

基金机构："National Natural Science Foundation of China [81100905]; Natural Science Foundation of Chongqing, Chongqing, China [Cstc2017jcyjAX0211]"

基金资助正文："Acknowledgements This work was supported by the National Natural Science Foundation of China (81100905) , the Natural Science Foundation of Chongqing, Chongqing, China (Cstc2017jcyjAX0211) ."