A highly sensitive and specific fluorescent strategy for the detection of Visfatin based on nonlinear hybridization chain reaction

作者全名："Liu, Feifei; Yuan, Yin; Zhang, Wanliang; Fu, Yafei; Yang, Mengliu; Yang, Gangyi; Liu, Hua; Shen, Huawei; Li, Ling"

作者地址："[Liu, Feifei; Yuan, Yin; Zhang, Wanliang; Fu, Yafei; Li, Ling] Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Lab Med Diagnost, Chongqing, Peoples R China; [Liu, Feifei; Yuan, Yin; Zhang, Wanliang; Fu, Yafei; Li, Ling] Chongqing Med Univ, Coll Lab Med, Dept Clin Biochem, Chongqing, Peoples R China; [Yang, Mengliu; Yang, Gangyi] Chongqing Med Univ, Affiliated Hosp 2, Dept Endocrinol, Chongqing, Peoples R China; [Liu, Hua] Univ Mississippi, Med Ctr, Dept Pediat, 2500 North State St, Jackson, MS 39216 USA; [Shen, Huawei] Chongqing Hosp Tradit Chinese Med, Chongqing, Peoples R China"

通信作者："Li, L (通讯作者)，Chongqing Med Univ, Coll Lab Med, Minist Educ, Key Lab Lab Med Diagnost, Chongqing, Peoples R China.; Li, L (通讯作者)，Chongqing Med Univ, Coll Lab Med, Dept Clin Biochem, Chongqing, Peoples R China.; Shen, HW (通讯作者)，Chongqing Hosp Tradit Chinese Med, Chongqing, Peoples R China."

来源：MICROCHEMICAL JOURNAL

ESI学科分类：CHEMISTRY

WOS号：WOS:000975964400001

JCR分区：Q1

影响因子：4.9

年份：2023

卷号：190

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Fluorescent dendritic nanostructures; Nonlinear hybridization chain reaction; Aptamer; Fluorescent detection strategy; Visfatin

摘要："This study established a nonlinear hybridization chain reaction system in which trigger DNA initiates self-sustained assembly of double-stranded hairpin substrates labelled with Sulfo-Cyanine3 (Cy-3) into fluorescent dendritic nanostructures for visfatin detection. The trigger DNA chain initially bound with the anti-visfatin aptamer will be freed with visfatin, which induces continuous chain branching growth and results in an expo-nential increase in fluorescence signals. Under optimal conditions, the fluorescence intensity increased linearly with visfatin concentrations ranging from 1 ng mL-1 to 100 ng mL-1 (the limit of detection dropped to 0.028 ng mL-1). Furthermore, our study showed that magnetic bead separation minimizes the nonspecific signal of the method. The specificity, stability, and sensitivity of this method were also tested in the study. The results revealed excellent specificity, stability and sensitivity. In the aspect of application evaluation, we tested visfatin in clinical serum samples and obtained credible and accordant results so we suppose that our detection method might have bright prospects in biological molecule technology and early clinical discovery of type 2 diabetes (T2DM)."

基金机构："General Project of Chongqing Natural Science Foundation [cstc2020jcyj-msxmX0040]; National Natural Science Foundation of China [82170816, 81902171]"

基金资助正文："Acknowledgements The study was supported by grants from General Project of Chongqing Natural Science Foundation [cstc2020jcyj-msxmX0040] and the National Natural Science Foundation of China [Nos. 82170816, 81902171] ."