Wrinkled topography regulates osteogenesis via autophagy-mediated Wnt/beta-catenin signaling pathway in MC3T3-E1 cells

作者全名："Zhang, Qiang; Zhang, Qi; Yan, Xiao; Wang, Liping; Yuan, Xiao"

作者地址："[Zhang, Qiang; Zhang, Qi; Yan, Xiao; Yuan, Xiao] Qingdao Univ, Dept Orthodont, Affiliated Hosp, Qingdao 266003, Peoples R China; [Zhang, Qiang; Yan, Xiao; Yuan, Xiao] Qingdao Univ, Sch Stomatol, Qingdao 266003, Peoples R China; [Zhang, Qi] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Stomatol Hosp, Chongqing 401174, Peoples R China; [Wang, Liping] Qingdao Univ, Qingdao Stomatol Hosp Affiliated, Qingdao 266003, Peoples R China; [Wang, Liping] Qingdao Univ, Qingdao Stomatol Hosp Affiliated, 17 Dexian Rd, Qingdao, Shandong, Peoples R China; [Yuan, Xiao] Qingdao Univ, Dept Orthodont, Affiliated Hosp, Haier Rd 59, Qingdao 266000, Shandong, Peoples R China"

通信作者："Wang, LP (通讯作者)，Qingdao Univ, Qingdao Stomatol Hosp Affiliated, 17 Dexian Rd, Qingdao, Shandong, Peoples R China.; Yuan, X (通讯作者)，Qingdao Univ, Dept Orthodont, Affiliated Hosp, Haier Rd 59, Qingdao 266000, Shandong, Peoples R China."

来源：ARCHIVES OF ORAL BIOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000986178000001

JCR分区：Q2

影响因子：2.2

年份：2023

卷号：151

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Wrinkled topography; Osteogenesis; Wnt/beta-catenin; Autophagy; PDMS

摘要："Objective: In this study, we aimed to evaluate the effects of different dimensional wrinkled in topography on the osteogenic differentiation of MC3T3-E1 cells and explored the underlying mechanisms. Design: Polydimethylsiloxane (PDMS) with a wrinkled topography was synthesized using an elastomer base and crosslinking while observing by atomic force microscopy. MC3T3-E1 proliferation was detected by Cell Counting Kit-8(CCK-8) assays and the cell morphology was determined by phalloidin staining. Osteogenetic genes expression levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. In addition, Autophagy-related genes expression levels were evaluated by immunostaining and western blotting in MC3T3-E1 in order to assess the induction of autophagy. Results: In this experiment, the 0.7 mu m amplitude and 3 mu m wavelength (W3) group increased the expression of osteogenic markers, whereas the 4.3 mu m amplitude and 27 mu m wavelength (W27) group showed inhibition. Both the cytoplasm and the nucleus of beta-catenin, compared with those of the Flat, W3 increased, whereas W27 decreased. At the same time, the autophagy was consistent with the influence of the topography on osteogenic differentiation. Moreover, using CQ or RAPA significantly inhibited or promoted autophagy, as well as partially decreasing or increasing osteogenesis, respectively. Infecting siRNA-beta-catenin decreased the expression of RUNX2 and OSX in MC3T3-E1 cells both treated with CQ and RAPA. Conclusions: Wrinkled topographies activated the autophagy-mediated Wnt/beta-catenin signaling pathway and affected the osteogenic differentiation of MC3T3-E1 cells. The introduction of aligned topographies on biomaterial scaffolds could provide physical cues with which modulate MC3T3-E1 responses for bone engineering constructs."

基金机构：National Natural Science Founda-tion of China [32171303]; Qingdao Key Health Discipline Development Fund [22-3-7-lczx-7-nsh]

基金资助正文：Fundings This study was supported by the National Natural Science Founda-tion of China (Grant No. 32171303) and Qingdao Key Health Discipline Development Fund (Grant No. 22-3-7-lczx-7-nsh) .