Anti-inflammatory Effect of Low-Intensity Ultrasound in Septic Rats

作者全名："Tang, Wentao; Xia, Yi; Deng, Juan; Xu, Haopeng; Tang, Yilin; Xiao, Xinfang; Wu, Liu; Song, Guolin; Qin, Juan; Wang, Yan"

作者地址："[Tang, Wentao; Xia, Yi; Deng, Juan; Xu, Haopeng; Tang, Yilin; Xiao, Xinfang; Wu, Liu; Wang, Yan] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing Key Lab Biomed Engn, Chongqing, Peoples R China; [Song, Guolin] Guizhou Univ Tradit Chinese Med, Affiliated Hosp 2, Dept Emergency, Guiyang, Guizhou, Peoples R China; [Qin, Juan] Guizhou Med Univ, Guiyang Maternal & Child Hlth Care Hosp, Dept Obstet & Gynecol, Guiyang, Guizhou, Peoples R China; [Wang, Yan] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing Key Labo of Biomed Engn, Chongqing 400016, Peoples R China; [Qin, Juan] Guizhou Med Univ, Guiyang Maternal & Child Hlth Care Hosp, Dept Obstet & Gynecol, Guiyang 550003, Guizhou, Peoples R China; [Song, Guolin] Guizhou Univ Tradit Chinese Med, Affiliated Hosp 2, Dept Emergency, Guiyang 550003, Guizhou, Peoples R China"

通信作者："Wang, Y (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing Key Labo of Biomed Engn, Chongqing 400016, Peoples R China.; Qin, J (通讯作者)，Guizhou Med Univ, Guiyang Maternal & Child Hlth Care Hosp, Dept Obstet & Gynecol, Guiyang 550003, Guizhou, Peoples R China.; Song, GL (通讯作者)，Guizhou Univ Tradit Chinese Med, Affiliated Hosp 2, Dept Emergency, Guiyang 550003, Guizhou, Peoples R China."

来源：ULTRASOUND IN MEDICINE AND BIOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001001238600001

JCR分区：Q2

影响因子：2.4

年份：2023

卷号：49

期号：7

开始页：1602

结束页：1610

文献类型：Article

关键词：Sepsis; Low -intensity ultrasound; Cytokine storm; Interleukin 1 j3; Nuclear factor -KB signaling pathway

摘要："Objective: Sepsis is a severe systemic inflammatory response caused by infection. Here, the spleen region of Sprague-Dawley (SD) rats with sepsis was irradiated with low-intensity ultrasound (LIUS) to explore the regula-tion of inflammation and its mechanism by LIUS.Methods: In this study, 30 rats used for survival analysis were randomly divided into the sham-operated group (Sham, n = 10), the group in which sepsis was induced by cecal ligation and puncture (CLP, n = 10) and the group treated with LIUS immediately after CLP (LIUS, n = 10). The other 120 rats were randomly divided into the aforementioned three groups for detection at each time point. The parameters used in the LIUS group were 200 mW/cm2, 0.37 MHz, 20% duty cycle and 20 min, and no ultrasonic energy was produced in the Sham and CLP groups. Seven-day survival rate, histopathology and expression of inflammatory factors and proteins were evaluated in the three groups.Results: LIUS was able to improve the survival rate of septic SD rats (p < 0.05), significantly inhibit the expression of tumor necrosis factor a (TNF-a), interleukin 1 j3 (IL-1 j3), interleukin 6 (IL-6) and nuclear factor-KB p65 (NF-KB p65) (p < 0.05) and restore the ultrastructure of the spleen.Conclusion: Our study determined that LIUS can relieve spleen damage and alleviate severe cytokine storm to improve survival outcomes in septic SD rats, and its mechanism may be related to the inhibition of the NF-KB sig-naling pathway by downregulation of IL-1 j3."

基金机构："Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0155]"

基金资助正文："Funding This work was supported by the Program for Youth Innovation in Future Medicine, Chongqing Medical University (W0155) ."