Oncostatin M regulates macrophages polarization in osseointegration via yes-associated protein

作者全名："Yuan, Ying; Zhang, Qin; Wu, Bingfeng; Huang, Tianyu; Gong, Ping; Xiang, Lin"

作者地址："[Yuan, Ying; Zhang, Qin; Gong, Ping; Xiang, Lin] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis & Natl Clin Res Ctr Oral Di, Chengdu, Peoples R China; [Yuan, Ying; Zhang, Qin; Gong, Ping; Xiang, Lin] Sichuan Univ, Dept Oral Implantol, Chengdu, Peoples R China; [Yuan, Ying] Chongqing Med Univ, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 400016, Peoples R China; [Wu, Bingfeng; Huang, Tianyu] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis & Natl Clin Res Ctr Oral Di, Chengdu, Peoples R China; [Xiang, Lin] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis & Natl Clin Res Ctr Oral Di, 14 Third Sect,Renmin Nan Rd, Chengdu 610041, Peoples R China; [Xiang, Lin] Sichuan Univ, Dept Oral Implantol, 14 Third Sect,Renmin Nan Rd, Chengdu 610041, Peoples R China"

通信作者："Xiang, L (通讯作者)，Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis & Natl Clin Res Ctr Oral Di, 14 Third Sect,Renmin Nan Rd, Chengdu 610041, Peoples R China.; Xiang, L (通讯作者)，Sichuan Univ, Dept Oral Implantol, 14 Third Sect,Renmin Nan Rd, Chengdu 610041, Peoples R China."

来源：INTERNATIONAL IMMUNOPHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001008760300001

JCR分区：Q1

影响因子：4.8

年份：2023

卷号：120

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Bone remodeling; regeneration; Bone biology; Dental implant(s); Immunity; Cell signaling

摘要："Objectives: Oncostatin M(OSM), secreted by monocytes and macrophages, has been noted to participate in bone homeostasis and macrophage polarization, which might be regulated by yes-associated protein (YAP). This study aimed to elucidate the influence and mechanisms of OSM-YAP on macrophages polarization in osseointegration. Material and methods: In vitro, flow cytometry, real-time PCR, and Elisa were performed to evaluate inflammatory function in bone marrow-derived macrophages (BMDMs) with OSM, siOSMR, and YAP inhibitor verteporfin (VP). In vivo, macrophage-specific YAP-deficient mice were generated to investigate the role of OSM via YAP signaling in osseointegration. Results: This study demonstrated that OSM could inhibit the M1 polarization, promote the M2 polarization, and induce the expression of osteogenic-related factors via VP. The conditional knock-out of YAP inhibited the osseointegration in mice, and promoted the inflammatory reaction around the implants, while OSM could restore the effect. Conclusions: Our results demonstrated that OSM might play an important role in the polarization of BMDMs, and bone formation around dental and femoral implants. This effect was closely conducted by Hippo-YAP pathway. Clinical Significance: Understanding the role and mechanism of OSM in macrophage polarization around dental implants could improve comprehension of signal network of osseointegration, and it might offer a potential target of therapies to accelerate osseointegration and reduce inflammatory reactions."

基金机构："National Natural Science Foundation of China [82170997, 82170935]; Project of Chengdu Science and Technology Bureau [2021-YF05-02054-SN]"

基金资助正文："This work was supported by grants from the National Natural Science Foundation of China (No.82170997, 82170935) , Project of Chengdu Science and Technology Bureau (No.2021-YF05-02054-SN) ."