Increased RNA editing sites revealed as potential novel biomarkers for diagnosis in primary Sjogren's syndrome

作者全名："Wang, Xiaobing; Zhu, Lingxiao; Ying, Senhong; Liao, Xin; Zheng, Junjie; Liu, Zhenwei; Gao, Jianxia; Niu, Miaomiao; Xu, Xin; Zhou, Zihao; Xu, Huji; Wu, Jinyu"

作者地址："[Wang, Xiaobing; Xu, Huji] Naval Med Univ, Shanghai Changzheng Hosp, Dept Rheumatol & Immunol, Shanghai 200003, Peoples R China; [Zhu, Lingxiao; Zheng, Junjie] Wenzhou Med Univ, Affiliated Hosp 1, Dept Rheumatol & Immunol, Wenzhou, Peoples R China; [Ying, Senhong] Chongqing Med Univ, Affiliated Hosp 2, Precis Med Ctr, Chongqing, Peoples R China; [Liao, Xin] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing, Peoples R China; [Liu, Zhenwei; Gao, Jianxia; Wu, Jinyu] Wenzhou Med Univ, Inst Genom Med, Wenzhou 325000, Peoples R China; [Niu, Miaomiao] Ningbo Hlth Gene Technol Co, Ningbo, Peoples R China; [Xu, Xin] Shandong Canc Hosp & Inst, Jinan, Peoples R China; [Zhou, Zihao] Southern Univ Sci & Technol, Peoples Hosp Shenzhen 3, Natl Clin Res Ctr Infect Dis, Dept Clin Lab, Shenzhen, Peoples R China; [Xu, Huji] Tsinghua Univ, Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China; [Xu, Huji] Tsinghua Univ, Sch Clin Med, Beijing, Peoples R China"

通信作者："Xu, HJ (通讯作者)，Naval Med Univ, Shanghai Changzheng Hosp, Dept Rheumatol & Immunol, Shanghai 200003, Peoples R China.; Wu, JY (通讯作者)，Wenzhou Med Univ, Inst Genom Med, Wenzhou 325000, Peoples R China."

来源：JOURNAL OF AUTOIMMUNITY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001009727700001

JCR分区：Q1

影响因子：7.9

年份：2023

卷号：138

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Primary Sjo center dot gren 's syndrome; RNA editing; ADAR1; RNA sequencing; Diagnosis

摘要："Background: Transcriptome-wide aberrant RNA editing has been shown to contribute to autoimmune diseases, but its extent and significance in primary Sjo center dot gren's syndrome (pSS) are currently poorly understood.Methods: We systematically characterized the global pattern and clinical relevance of RNA editing in pSS by performing large-scale RNA sequencing of minor salivary gland tissues obtained from 439 pSS patients and 130 non-pSS or healthy controls.Findings: Compared with controls, pSS patients displayed increased global RNA-editing levels, which were significantly correlated and clinically relevant to various immune features in pSS. The elevated editing levels were likely explained by significantly increased expression of adenosine deaminase acting on RNA 1 (ADAR1) p150 in pSS, which was associated with disease features. In addition, genome-wide differential RNA editing (DRE) analysis between pSS and non-pSS showed that most (249/284) DRE sites were hyper-edited in pSS, especially the top 10 DRE sites dominated by hyper-edited sites and assigned to nine unique genes involved in the inflammatory response or immune system. Interestingly, among all DRE sites, six RNA editing sites were only detected in pSS and resided in three unique genes (NLRC5, IKZF3 and JAK3). Furthermore, these six specific DRE sites with significant clinical relevance in pSS showed a strong capacity to distinguish between pSS and non-pSS, reflecting powerful diagnostic efficacy and accuracy.Conclusion: These findings reveal the potential role of RNA editing in contributing to the risk of pSS and further highlight the important prognostic value and diagnostic potential of RNA editing in pSS."

基金机构："National Natural Science Foundation of China [82271816, 32070594]; National Key Research and Development Project [2018AAA0100302]; Shanghai Municipal Key Clinical Specialty [shslczdzk02602]; Zhejiang Provincial Natural Science Foundation of China [LY23C060002]"

基金资助正文："This work was supported by the National Natural Science Foundation of China (Grant No. 82271816 & 32070594) , National Key Research and Development Project (Grant No. 2018AAA0100302) , Shanghai Municipal Key Clinical Specialty (shslczdzk02602) , Zhejiang Provincial Natural Science Foundation of China (Grant No. LY23C060002) ."