Mechanism of M2 macrophages modulating astrocyte polarization through the TGF-8/PI3K/Akt pathway

作者全名："Pang, Qi-Ming; Zhang, Qian; Wu, Xiang-Chong; Yang, Rui-Lin; Fu, Sheng-Ping; Fan, Zhen-Hai; Liu, Juan; Yu, Li-Mei; Peng, Jia-Chen; Zhang, Tao"

作者地址："[Pang, Qi-Ming; Yang, Rui-Lin; Fan, Zhen-Hai; Liu, Juan; Yu, Li-Mei; Zhang, Tao] Zunyi Med Univ, Key Lab Cell Engn Guizhou Prov, Affiliated Hosp, Zunyi, Guizhou, Peoples R China; [Pang, Qi-Ming; Wu, Xiang-Chong; Fu, Sheng-Ping; Peng, Jia-Chen; Zhang, Tao] Zunyi Med Univ, Dept Orthopaed Surg, Affiliated Hosp, Zunyi, Guizhou, Peoples R China; [Pang, Qi-Ming] Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 1, Chongqing, Peoples R China; [Zhang, Qian] Zunyi Med Univ, Dept Human Anat, Zunyi, Guizhou, Peoples R China"

通信作者："Zhang, T (通讯作者)，Zunyi Med Univ, Key Lab Cell Engn Guizhou Prov, Affiliated Hosp, Zunyi, Guizhou, Peoples R China.; Peng, JC; Zhang, T (通讯作者)，Zunyi Med Univ, Dept Orthopaed Surg, Affiliated Hosp, Zunyi, Guizhou, Peoples R China."

来源：IMMUNOLOGY LETTERS

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001010507900001

JCR分区：Q3

影响因子：3.3

年份：2023

卷号：259

期号：　

开始页：1

结束页：8

文献类型：Article

关键词：Spinal cord injury; Astrocyte; Macrophage; Neuroinflammation

摘要："Recent studies have revealed that activated astrocytes (AS) are divided into two distinct types, termed A1 and A2. A2 astrocytes are neuroprotective and promote tissue repair and regeneration following spinal cord injury. Whereas, the specific mechanism for the formation of the A2 phenotype remains unclear. This study focused on the PI3K/Akt pathway and examined whether TGF-8 secreted by M2 macrophages could mediate A2 polarization by activating this pathway. In this study, we revealed that both M2 macrophages and their conditioned medium (M2-CM) could facilitate the secretion of IL-10, IL-13 and TGF-8 from AS, and this effect was significantly reversed after the administration of SB431542 (a TGF-8 receptor inhibitor) or LY294002 (a PI3K inhibitor). Moreover, immunofluorescence results demonstrated that TGF-8 secreted by M2 macrophages could facilitate the expression of A2 biomarker S100A10 in AS; combined with the results of western blot, it was found that this effect was closely related to the activation of PI3K/Akt pathway in AS. In conclusion, TGF-8 secreted by M2 macrophages may induce the conversion of AS to the A2 phenotype through the activation of the PI3K/Akt pathway."

基金机构："National Natural Science Foundation of China [81960299, 82160599]"

基金资助正文："This work was supported by grants from the National Natural Science Foundation of China (No. 81960299, No.82160599) ."