Salidroside pretreatment alleviates PM2.5 caused lung injury via inhibition of apoptosis and pyroptosis through regulating NLRP3 Inflammasome

作者全名："Shi, Shihua; Huang, Demei; Wu, Yongcan; Pei, Caixia; Wang, Yilan; Shen, Zherui; Zhao, Sijing; Jia, Nan; Wang, Xiaomin; Chen, Bonan; Pan, Jie; Wang, Fei; Wang, Zhenxing"

作者地址："[Shi, Shihua; Huang, Demei; Pei, Caixia; Wang, Yilan; Shen, Zherui; Zhao, Sijing; Jia, Nan; Wang, Xiaomin; Wang, Fei; Wang, Zhenxing] Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610072, Peoples R China; [Shi, Shihua] Swiss Trop & Publ Hlth Inst, Dept Epidemiol & Publ Hlth, CH-4123 Basel, Switzerland; [Shi, Shihua] Univ Basel, Fac Sci, CH-4058 Basel, Switzerland; [Wu, Yongcan] Chongqing Key Lab Tradit Chinese Med Prevent & Cur, Chongqing 400016, Peoples R China; [Wu, Yongcan] Chongqing Med Univ, Coll Tradit Chinese Med, Chongqing 400016, Peoples R China; [Chen, Bonan] Chinese Univ Hong Kong, Prince Wales Hosp, Dept Anat & Cellular Pathol, State Key Lab Translat Oncol, Hong Kong 99907, Peoples R China; [Pan, Jie] Stanford Univ, Dept Pathol, Sch Med, Palo Alto, CA 94305 USA"

通信作者："Wang, F; Wang, ZX (通讯作者)，Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610072, Peoples R China."

来源：FOOD AND CHEMICAL TOXICOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:001013395600001

JCR分区：Q1

影响因子：3.9

年份：2023

卷号：177

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Salidroside; PM2; 5 caused lung injury; Apoptosis; Pyroptosis; NLRP3 inflammasome

摘要："Ambient fine particulate matter (PM2.5) is considered a leading cause of pathogenic particulate matter induced lung injury. And Salidroside (Sal), the major bioactive constituent isolated from Rhodiola rosea L., has been shown to ameliorate lung injury in various conditions. To uncover the possible therapy for PM2.5 related pulmonary dis-ease, we evaluated the protective role of Sal pre-treatment on PM2.5 induced lung injury in mice by utilizing the survival analysis, hematoxylin and eosin (H & E) staining, lung injury score, lung wet-to-dry weight ratio, enzyme -linked immunosorbent assay (ELISA) kits, immunoblot, immunofluorescence, and transmission electron micro-scopy (TEM). Impressively, our findings strongly indicated Sal as an effective precaution against PM2.5 induced lung injury. Pre-administration of Sal before PM2.5 treatment reduced the mortality within 120 h and alleviated inflammatory responses by reducing the release of proinflammatory cytokines, including TNF-& alpha;, IL-1 & beta;, and IL-18. Meanwhile, Sal pretreatment blocked apoptosis and pyroptosis that introduced the tissue damage under PM2.5 treatment via regulating Bax/Bcl-2/caspase-3 and NF-cB/NLRP3/caspase-1 signal pathways. In summary, our research demonstrated that Sal could be a potential preventative therapy for PM2.5 caused lung injury by inhibiting the initiation and development of apoptosis and pyroptosis through down-regulating NLRP3 inflam-masome pathway."

基金机构：China Postdoctoral Science Foundation [2020M683645XB]

基金资助正文："This research was funded by the China Postdoctoral Science Foundation (No. 2020M683645XB). We greatly appreciate the pioneer of ""WE"" medicine, Prof. Yung-Chi Cheng of Yale School of Medicine. We thank him for the help and support for Shihua Shi's study."