A polyaniline functionalized NiFeP nanosheet array-based electrochemical immunosensor using Au/Cu2O nanocubes as a signal amplifier for the detection of SARS-CoV-2 nucleocapsid protein

作者全名："Bai, Liwei; Shi, Yufen; Zhang, Xue; Cao, Xiaowei; Jia, Jianhua; Shi, Huanhuan; Lu, Wenbo"

作者地址："[Bai, Liwei; Shi, Yufen; Zhang, Xue; Lu, Wenbo] Shanxi Normal Univ, Sch Chem & Mat Sci, Key Lab Magnet Mol & Magnet Informat Mat, Minist Educ, Taiyuan 030031, Peoples R China; [Shi, Huanhuan] Karlsruher Inst Technol, Inst Quanten Mat & Technol, Hermann v Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany; [Cao, Xiaowei] Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R China; [Jia, Jianhua] Chongqing Med Univ, Coll Basic Med Sci, Chongqing 400016, Peoples R China"

通信作者："Lu, WB (通讯作者)，Shanxi Normal Univ, Sch Chem & Mat Sci, Key Lab Magnet Mol & Magnet Informat Mat, Minist Educ, Taiyuan 030031, Peoples R China.; Shi, HH (通讯作者)，Karlsruher Inst Technol, Inst Quanten Mat & Technol, Hermann v Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany."

来源：ANALYST

ESI学科分类：CHEMISTRY

WOS号：WOS:001016646200001

JCR分区：Q2

影响因子：3.6

年份：2023

卷号：148

期号：14

开始页：3359

结束页：3370

文献类型：Article

关键词：　

摘要："Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged as a novel pathogen in 2019. The virus is responsible for a severe acute respiratory syndrome outbreak, affecting the respiratory system of infected individuals. COVID-19 is a super amplifier of basic diseases, and the disease with basic diseases is often more serious. Controlling the spread of the COVID-19 pandemic relies heavily on the timely and accurate detection of the virus. To resolve the problem, a polyaniline functionalized NiFeP nanosheet array-based electrochemical immunosensor using Au/Cu2O nanocubes as a signal amplifier is fabricated for the detection of SARS-CoV-2 nucleocapsid protein (SARS-CoV-2 NP). Polyaniline (PANI) functionalized NiFeP nanosheet arrays are synthesized as an ideal sensing platform for the first time. PANI is coated on the surface of NiFeP by electropolymerization to enhance biocompatibility, beneficial for the efficient loading of the capture antibody (Ab(1)). Significantly, Au/Cu2O nanocubes possess excellent peroxidase-like activity and exhibit outstanding catalytic activity for the reduction of H2O2. Therefore, Au/Cu2O nanocubes combine with a labeled antibody (Ab(2)) through the Au-N bond to form labeled probes, which can effectively amplify current signals. Under optimal conditions, the immunosensor for the detection of SARS-CoV-2 NP shows a wide linear range of 10 fg mL(-1)-20 ng mL(-1) and a low detection limit of 1.12 fg mL(-1) (S/N = 3). It also exhibits desirable selectivity, repeatability, and stability. Meanwhile, the excellent analytical performance in human serum samples confirms the practicality of the PANI functionalized NiFeP nanosheet array-based immunosensor. The electrochemical immunosensor based on the Au/Cu2O nanocubes as a signal amplifier demonstrates great potential for application in the personalized point-of-care (POC) clinical diagnosis."

基金机构：National Natural Science Foundation of China [21705103]; Applied Basic Research Project of Shanxi Province [202103021224251]; Scientific and Technological Innovation Projects in Shanxi Universities [2019L0460]; Graduate Education Innovation Project of Shanxi Province [2021Y485]; 1331 Engineering of Shanxi Province

基金资助正文："This work was supported by the National Natural Science Foundation of China (no. 21705103), the Applied Basic Research Project of Shanxi Province (no. 202103021224251), the Scientific and Technological Innovation Projects in Shanxi Universities (no. 2019L0460), the Graduate Education Innovation Project of Shanxi Province (2021Y485), and the 1331 Engineering of Shanxi Province. Human blood samples are provided by the Linfen People's Hospital (Binghe West Road, Yaodu District, Linfen, Shanxi, China)."