Interleukin-4 Promotes Human Metapneumovirus Replication Through the JAK/STAT6 Pathway

作者全名："Zhang, Yueyan; Wu, Guojin; Yang, Yuting; Niu, Linlin; Zhao, Yao"

作者地址："[Zhang, Yueyan; Wu, Guojin; Yang, Yuting; Niu, Linlin; Zhao, Yao] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ Key Lab Child Dev & Disorders, Chongqing Key Lab Child Infect & Immun,China Int S, Chongqing, Peoples R China; [Zhao, Yao] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ Key Lab Child Dev & Disorders, Chongqing Key Lab Child Infect & Immun,China Int S, 136 Zhongshan Erlu, Chongqing 400014, Peoples R China"

通信作者："Zhao, Y (通讯作者)，Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ Key Lab Child Dev & Disorders, Chongqing Key Lab Child Infect & Immun,China Int S, 136 Zhongshan Erlu, Chongqing 400014, Peoples R China."

来源：VIRAL IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001022093500001

JCR分区：Q4

影响因子：1.5

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：human metapneumovirus; children; IL-4; viral replication; JAK; STAT6 pathway

摘要："Respiratory virus infections are the main causes of pediatric diseases. Human metapneumovirus (hMPV) is an enveloped RNA virus similar to severe acute respiratory syndrome coronavirus type 2, both of which have emerged as important new respiratory viruses. Recent studies have found that interleukin-4 (IL-4) is involved in the replication of a variety of viruses, and its role differs in different viruses. The purpose of this study was to investigate the effect of IL-4 on hMPV and to elucidate its mechanism of action. We found that hMPV infection promoted the expression of IL-4 in human bronchial epithelial cells. The replication of the virus was reduced using small interfering RNA knockdown of IL-4 expression, while the addition of exogenous recombinant human IL-4 to IL-4 knockdown cells restored viral replication ability. These results demonstrate that the expression of IL-4 is closely related to the replication of hMPV; moreover, further experiments revealed that IL-4 promotes the replication of hMPV through a mechanism dependent on the Janus kinase/signal transductor and transcription activator 6 signaling pathway. Therefore, anti-IL-4 strategies may be a promising avenue for the treatment of hMPV infection, representing an important breakthrough for children at risk from hMPV infection."

基金机构：Chongqing Talent Program [CQYC20210303393]; Program for Youth Innovation in Future Medicine at Chongqing Medical University; National Natural Science Foundation of China [81701997]; Natural Science Foundation Project of CQ [cstc2019jcyj-msxmX0244]

基金资助正文："This study was supported by the Chongqing Talent Program (CQYC20210303393), the Program for Youth Innovation in Future Medicine at Chongqing Medical University, National Natural Science Foundation of China (81701997), Natural Science Foundation Project of CQ (cstc2019jcyj-msxmX0244)."