Biomimetic Fibers Derived from an Equidistant Micropillar Platform Dictate Osteocyte Fate via Mechanoreception

作者全名："Liu, Yang; Pu, Xiaohua; Duan, Mengmeng; Chen, Cheng; Zhao, Yanfang; Zhang, Demao; Xie, Jing"

作者地址："[Liu, Yang; Pu, Xiaohua; Duan, Mengmeng; Zhang, Demao; Xie, Jing] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610044, Peoples R China; [Chen, Cheng] Chongqing Med Univ, Coll Med Informat, Chongqing 400016, Peoples R China; [Zhao, Yanfang] Univ Alabama Birmingham, Sch Dent, Dept Pediat Dent, Birmingham, AL 35233 USA; [Zhang, Demao] Sichuan Univ, Inst Biomed Engn, West China Sch Basic Med Sci & Forens Med, Chengdu 610041, Peoples R China; [Xie, Jing] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu 610044, Peoples R China"

通信作者："Xie, J (通讯作者)，Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610044, Peoples R China.; Xie, J (通讯作者)，Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu 610044, Peoples R China."

来源：NANO LETTERS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001022366300001

JCR分区：Q1

影响因子：9.6

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Micropillar substrate; ECM stiffness; Osteocytes; Synaptogenesis; Mechanoreception; Mechanotransduction

摘要："It is a big challenge to design a biomimetic physicalmicroenvironmentwith greater similarity to in vivo tissue to observe real cell behaviors.We established a novel cell culture platform based on patterned equidistantmicropillars with stiff and soft stiffnesses to mimic the changesthat happened in the transition from normal to osteoporotic disease.We first demonstrated that the soft micropillar substrate decreasedosteocyte synaptogenesis through synaptogyrin 1 and that this decreasewas accompanied by impairment of cell mechanoperception and a decreasein cellular cytoskeletal rearrangement. We then found that the softequidistant micropillar substrate reduced the osteocyte synaptogenesismainly via the inactivation of Erk/MAPK signaling. We finally foundthat soft micropillar substrate-mediated synaptogenesis impacted thecell-to-cell communication and matrix mineralization of osteocytes.Taken together, this study provides evidence of cellular mechanicalresponses that are much more similar to those of real osteocytes atthe bone tissue level."

基金机构："National Natural Science Foundation of China [81771047, 22CXRC0216]"

基金资助正文：This work was supported by the National Natural Science Foundation of China (81771047 and 22CXRC0216 to J.X.).