Establishment of a Rat Model of Capillary Leakage Syndrome Induced by Cardiopulmonary Resuscitation After Cardiac Arrest

作者全名："Zhang, Xiao-lei; Cheng, Ye; Xing, Chun-lin; Ying, Jia-yun; Yang, Xue; Cai, Xiao-di; Lu, Guo-ping"

作者地址："[Zhang, Xiao-lei; Cheng, Ye; Ying, Jia-yun; Yang, Xue; Cai, Xiao-di; Lu, Guo-ping] Fudan Univ, Childrens Hosp, Dept Pediat Emergency Med & Crit Care Med, Shanghai 201102, Peoples R China; [Xing, Chun-lin] Chongqing Med Univ, Childrens Hosp, Dept Pediat Crit Care Med, Chongqing 400014, Peoples R China"

通信作者："Lu, GP (通讯作者)，Fudan Univ, Childrens Hosp, Dept Pediat Emergency Med & Crit Care Med, Shanghai 201102, Peoples R China."

来源：CURRENT MEDICAL SCIENCE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:001022538100003

JCR分区：Q3

影响因子：2

年份：2023

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期号：　

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结束页：　

文献类型：Article; Early Access

关键词：cardiac arrest; cardiopulmonary resuscitation; capillary leakage; model

摘要："ObjectiveCardiopulmonary resuscitation (CPR) after cardiac arrest (CA) is one of the main causes of capillary leakage syndrome (CLS). This study aimed to establish a stable CLS model following the CA and cardiopulmonary resuscitation (CA-CPR) model in Sprague-Dawley (SD) rats.MethodsWe conducted a prospective, randomized, animal model study. All adult male SD rats were randomly divided into a normal group (group N), a sham operation group (group S), and a cardiopulmonary resuscitation group (group T). The SD rats of the three groups were all inserted with 24-G needles through their left femoral arteries and right femoral veins. In group S and group T, the endotracheal tube was intubated. In group T, CA induced by asphyxia (AACA) was caused by vecuronium bromide with the endotracheal tube obstructed for 8 min, and the rats were resuscitated with manual chest compression and mechanical ventilation. Preresuscitation and postresuscitation measurements, including basic vital signs (BVS), blood gas analysis (BG), routine complete blood count (CBC), wet-to-dry ratio of tissues (W/D), and the HE staining results after 6 h were evaluated.ResultsIn group T, the success rate of the CA-CPR model was 60% (18/30), and CLS occurred in 26.6% (8/30) of the rats. There were no significant differences in the baseline characteristics, including BVS, BG, and CBC, among the three groups (P>0.05). Compared with pre-asphyxia, there were significant differences in BVS, CBC, and BG, including temperature, oxygen saturation (SpO(2)), mean arterial pressure (MAP), central venous pressure (CVP), white blood cell count (WBC), hemoglobin, hematocrit, pH, pCO(2), pO(2), SO2, lactate (Lac), base excess (BE), and Na+ (P<0.05) after the return of spontaneous circulation (ROSC) in group T. At 6 h after ROSC in group T and at 6 h after surgery in groups N and S, there were significant differences in temperature, heart rate (HR), respiratory rate (RR), SpO(2), MAP, CVP, WBC, pH, pCO(2), Na+, and K+ among the three groups (P<0.05). Compared with the other two groups, the rats in group T showed a significantly increased W/D weight ratio (P<0.05). The HE-stained sections showed consistent severe lesions in the lung, small intestine, and brain tissues of the rats at 6 h after ROSC following AACA.ConclusionThe CA-CPR model in SD rats induced by asphyxia could reproduce CLS with good stability and reproducibility."

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