Construction of Magnetic Core-Large Mesoporous Satellite Immunosensor for Long-Lasting Chemiluminescence and Highly Sensitive Tumor Marker Determination

作者全名："Liu, Shude; Li, Juan; Zou, Yidong; Jiang, Yongjian; Wu, Limin; Deng, Yonghui"

作者地址："[Liu, Shude; Li, Juan; Zou, Yidong; Deng, Yonghui] Fudan Univ, Zhongshan Hosp, Dept Chem, iChEM,Shanghai Key Lab Mol Catalysis & Innovat Mat, Shanghai 200433, Peoples R China; [Li, Juan] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Jiang, Yongjian] Fudan Univ, Huashan Hosp, Dept Pancreat Surg Nephrol & Radiol, Shanghai 200040, Peoples R China; [Wu, Limin] Inner Mongolia Univ, Inst Energy & Mat Chem, Hohhot 010021, Peoples R China"

通信作者："Li, J; Deng, YH (通讯作者)，Fudan Univ, Zhongshan Hosp, Dept Chem, iChEM,Shanghai Key Lab Mol Catalysis & Innovat Mat, Shanghai 200433, Peoples R China.; Li, J (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China.; Wu, LM (通讯作者)，Inner Mongolia Univ, Inst Energy & Mat Chem, Hohhot 010021, Peoples R China."

来源：SMALL

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001024046000001

JCR分区：Q1

影响因子：13

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：core-satellites; disease diagnosis; large-mesoporous; long-lasting chemiluminescence; magnetic microspheres

摘要："Chemiluminescence immunoassay exhibits high sensitivity and signal-to-noise ratio, thus attracting great attention in the early diagnosis and dynamic monitoring of diseases. However, the collection of conventional flash-type chemiluminescence signal (<5 s) relies heavily on automatic sampling and reading instrument. Herein, a novel core-satellite multifunctional chemiluminescence immunosensor is designed for the efficient enrichment and highly sensitive determination of cancer biomarker carcinoembryonic antigen (CEA) with enhanced and long-lasting output signal that can be conveniently recorded by a simple microplate plate reading instrument. Anti-CEA monoclonal antibody 2 (Ab2) modified Fe3O4@SiO2 microspheres (Fe3O4@SiO2-Ab2, 370 nm in diameter) are synthesized as the core for selectively capturing and enriching target CEA in solution, and anti-human CEA monoclonal antibody 1 (Ab1) and horseradish peroxidase (HRP) co-immobilized dendritic large-mesoporous silica nanospheres (MSNs-HRP/Ab1, 80 nm in diameter, pore size: 17 nm) are synthesized as the satellite for efficient immunological recognition and signal amplification. The as-designed core-satellite magnetic chemiluminescence immunosensors exhibit a broad linear range of 0.01-20 ng mL(-1) and a low detection limit of 3.0 pg mL(-1) for the convenient, highly specific, and sensitive determination of CEA in human serum. Such core-satellite chemiluminescence immunosensors are expected to act as a powerful tool for in vitro detection of various biomarkers, overcome the defect of conventional chemiluminescence relying heavily on expensive and bulky automatic instruments and popularize chemiluminescence analysis to primary medical institutions and remote areas."

基金机构："NSF of China [22125501, U22A20152]; Key Basic Research Program of Science and Technology Commission of Shanghai Municipality [20JC1415300]; Fundamental Research Funds for the Central Universities [20720220010]; Foshan Science and Technology Innovation Program [2017IT100121]"

基金资助正文："Acknowledgements This work was financially supported by NSF of China (22125501, U22A20152), Key Basic Research Program of Science and Technology Commission of Shanghai Municipality (20JC1415300), Fundamental Research Funds for the Central Universities (no. 20720220010), and Foshan Science and Technology Innovation Program (no. 2017IT100121)."