Hybridization altered the gut microbiota of pigs

作者全名："Wei, Limin; Zeng, Bo; Zhang, Siyuan; Guo, Wei; Li, Feng; Zhao, Jiangchao; Li, Ying"

作者地址："[Wei, Limin] Chongqing Med & Pharmaceut Coll, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing, Peoples R China; [Wei, Limin; Li, Ying] Foshan Univ, Coll Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise B, Foshan, Peoples R China; [Wei, Limin] Chongqing Med Univ, Coll Pharm, Chongqing, Peoples R China; [Zeng, Bo] Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key L, Chengdu, Peoples R China; [Zhang, Siyuan; Guo, Wei] Chengdu Med Coll, Sch Lab Med, Sichuan Prov Engn Lab Prevent & Control Technol Ve, Chengdu, Peoples R China; [Li, Feng] China West Normal Univ, Key Lab Southwest China Wildlife Resources Conserv, Minist Educ, Nanchong, Peoples R China; [Zhao, Jiangchao] Univ Arkansas, Dept Anim Sci, Div Agr, Fayetteville, AR USA"

通信作者："Li, Y (通讯作者)，Foshan Univ, Coll Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise B, Foshan, Peoples R China.; Zeng, B (通讯作者)，Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key L, Chengdu, Peoples R China."

来源：FRONTIERS IN MICROBIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:001026229300001

JCR分区：Q2

影响因子：4

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：gut microbiota; hybridization; pig; 16S rRNA; metagenome; metabolome

摘要："Mammalian gut microbiota plays an important role in the host's nutrient metabolism, growth, and immune regulation. Hybridization can enable a progeny to acquire superior traits of the parents, resulting in the hybridization advantage. However, studies on the effects of hybridization on the pigs' gut microbiota are lacking. Therefore, this study used multi-omics technologies to compare and analyze the gut microbiota of the primary wild boar and its offspring. The 16S rRNA gene sequencing results revealed that the gut microbiota of F4 exhibited a host-like dominance phenomenon with a significant increase in the abundance of Lactobacillus and Bifidobacterium. The beta diversity of Duroc was significantly different from those of F0, F2, and F4; after the host hybridization, the similarity of the beta diversity in the progeny decreased with the decrease in the similarity of the F0 lineage. The metagenomic sequencing results showed that the significantly enriched metabolic pathways in F4, such as environmental, circulatory system, fatty acid degradation adaptation, and fatty acid biosynthesis, were similar to those in F0. Moreover, it also exhibited similar significantly enriched metabolic pathways as those in Duroc, such as carbohydrate metabolism, starch and sucrose metabolism, starch-degrading CAZymes, lactose-degrading CAZymes, and various amino acid metabolism pathways. However, the alpha-amylase-related KOs, lipid metabolism, and galactose metabolism in F4 were significantly higher than those in Duroc and F0. Non-targeted metabolome technology analysis found that several metabolites, such as docosahexaenoic acid, arachidonic acid, and citric acid were significantly enriched in the F4 pigs as compared to those in F0. Based on Spearman correlation analysis, Lactobacillus and Bifidobacterium were significantly positively correlated with these metabolites. Finally, the combined metagenomic and metabolomic analysis suggested that the metabolic pathways, such as valine, leucine, and isoleucine biosynthesis and alanine aspartate and glutamate metabolism were significantly enriched in F4 pigs. In conclusion, the gut microbiota of F4 showed a similar host ""dominance"" phenomenon, which provided reference data for the genetics and evolution of microbiota and the theory of microbial-assisted breeding."

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