PARG Promotes Esophagus Cancer Cell Metastasis by Activation of the Wnt/ss-Catenin Pathway

作者全名："Yan, Jiaxin; Zhou, Yehan; Wang, Yalan; Liu, Yang"

作者地址："[Yan, Jiaxin; Zhou, Yehan; Liu, Yang] Sichuan Canc Hosp, Dept Pathol, 55 Renmin South Rd, Chengdu 610000, Sichuan, Peoples R China; [Wang, Yalan] Chongqing Med Univ, Sch Basic Med Sci, Dept Pathol, Chengdu, Peoples R China"

通信作者："Liu, Y (通讯作者)，Sichuan Canc Hosp, Dept Pathol, 55 Renmin South Rd, Chengdu 610000, Sichuan, Peoples R China."

来源：BIOCHEMICAL GENETICS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001026600800001

JCR分区：Q3

影响因子：2.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Esophagus cancer; PARG; Metastasis; Wnt/ss-catenin pathway

摘要："Esophagus cancer (EC) is a highly malignant and metastatic cancer. Poly(ADPribose) glycohydrolase (PARG), a DNA replication and repair regulator, inhibits cancer cell replication defects. This study aimed to explore the role of PARG in EC. The biological behaviors were analyzed using MTT assay, Transwell assay, scratch test, cell adhesion assay, and western blot. PARG expression was detected using quantitative PCR and immunohistochemical assay. The regulation of the Wnt/ss-catenin pathway was assessed using western blot. The results showed that PARG was highly expressed in EC tissues and cells. Knockdown of PARG suppressed cell viability, invasion, migration, adhesion, and epithelial-mesenchymal transition. Conversely, overexpression of PARG promoted the biological behaviors mentioned above. Moreover, overexpression of PARG promoted the activation of the Wnt/ss-catenin pathway rather than the STAT and Notch pathways. XAV939, the Wnt/ss-catenin pathway inhibitor, partly abolished the biological behaviors mediated by PARG overexpression. In conclusion, PARG promoted the malignant advancement of EC via activating the Wnt/ss-catenin pathway. These findings suggested that PARG might be a new therapeutic target for EC."

基金机构：Sichuan Provincial Medical Youth Innovation and Scientific Research Project Program [Q20030]

基金资助正文：& nbsp;The work was supported by Sichuan Provincial Medical Youth Innovation and Scientific Research Project Program (Grant No. Q20030).