Near-infrared fluorophore IR-61 delays postovulatory aging of mouse oocytes through suppressing oxidative stress mediated by mitochondrial protection

作者全名："Qu, Jiadan; Luo, Yunyao; Qin, Lifeng; Guo, Jing; Zhu, Ling; Li, Chong; Xie, Juan; Shi, Chunmeng; Huang, Guoning; Li, Jingyu"

作者地址："[Qu, Jiadan; Luo, Yunyao; Zhu, Ling; Li, Chong; Xie, Juan; Huang, Guoning; Li, Jingyu] Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Women & Childrens Hosp, Chongqing, Peoples R China; [Qu, Jiadan; Luo, Yunyao; Zhu, Ling; Li, Chong; Xie, Juan; Huang, Guoning; Li, Jingyu] Chongqing Hlth Ctr Women & Children, Chongqing Clin Res Ctr Reprod Med, Chongqing, Peoples R China; [Qin, Lifeng] Northeast Agr Univ, Coll Life Sci, Harbin, Peoples R China; [Guo, Jing] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun, Peoples R China; [Shi, Chunmeng] Third Mil Med Univ, Inst Rocket Force Med, State Key Lab Trauma Burns & Combined Injury, Chongqing, Peoples R China"

通信作者："Huang, GN; Li, JY (通讯作者)，Chongqing Med Univ, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Women & Childrens Hosp, Chongqing, Peoples R China."

来源：FASEB JOURNAL

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001032499700053

JCR分区：Q1

影响因子：4.4

年份：2023

卷号：37

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：IR-61; mitochondria; oocyte quality; oxidative stress; postovulatory aging

摘要："Postovulatory aging can trigger deterioration of oocyte quality and subsequent embryonic development, and thus reduce the success rates of assisted reproductive technology (ART). The molecular mechanisms underlying postovulatory aging, and preventative strategies, remain to be explored. The near-infrared fluorophore IR-61, a novel heptamethine cyanine dye, has the potential for mitochondrial targeting and cell protection. In this study, we found that IR-61 accumulated in oocyte mitochondria and reduced the postovulatory aging-induced decline in mitochondrial function, including mitochondrial distribution, membrane potential, mtDNA number, ATP levels, and mitochondrial ultrastructure. In addition, IR-61 rescued postovulatory aging-caused oocyte fragmentation, defects in spindle structure, and embryonic developmental potential. RNA sequencing analysis indicated that the postovulatory aging-induced oxidative stress pathway might be inhibited by IR-61. We then confirmed that IR-61 decreased the levels of reactive oxygen species and MitoSOX, and increased GSH content in aged oocytes. Collectively, the results indicate that IR-61 may prevent postovulatory aging by rescuing oocyte quality, promoting successful rate in ART procedure."

基金机构：National Natural Science Foundation of China [82101643]; Natural Science Foundation of Chongqing Province [CSTB2022NSCQ-MSX0961]

基金资助正文：ACKNOWLEDGMENTS We thank the research staff for their contributions to this study. This study was supported by the National Natural Science Foundation of China (82101643) and Natural Science Foundation of Chongqing Province (CSTB2022NSCQ-MSX0961).