Nanotube patterning reduces macrophage inflammatory response via nuclear mechanotransduction

作者全名："Fu, Yiru; Jing, Zheng; Chen, Tao; Xu, Xinxin; Wang, Xu; Ren, Mingxing; Wu, Yanqiu; Wu, Tianli; Li, Yuzhou; Zhang, He; Ji, Ping; Yang, Sheng"

作者地址："[Fu, Yiru; Jing, Zheng; Chen, Tao; Xu, Xinxin; Wang, Xu; Ren, Mingxing; Wu, Yanqiu; Wu, Tianli; Li, Yuzhou; Zhang, He; Ji, Ping; Yang, Sheng] Chongqing Med Univ, Coll Stomatol, 426 Songshi Bei Rd, Chongqing 401147, Peoples R China; [Jing, Zheng; Li, Yuzhou; Zhang, He; Ji, Ping; Yang, Sheng] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Jing, Zheng; Li, Yuzhou; Zhang, He; Ji, Ping; Yang, Sheng] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"

通信作者："Yang, S (通讯作者)，Chongqing Med Univ, Coll Stomatol, 426 Songshi Bei Rd, Chongqing 401147, Peoples R China.; Yang, S (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Yang, S (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China."

来源：JOURNAL OF NANOBIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:001033483600002

JCR分区：Q1

影响因子：10.6

年份：2023

卷号：21

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Titania nanotube patterning; Anodization; Mechanotransduction; Macrophage; Inflammatory response

摘要："The inflammatory immune environment surrounding titanium bone implants determines the formation of osseointegration, and nanopatterning on implant surfaces modulates the immune microenvironment in the implant region. Among many related mechanisms, the mechanism by which nanopatterning controls macrophage inflammatory response still needs to be elucidated. In this paper, we found that inhibition of the nuclear envelope protein lamin A/C by titania nanotubes (TNTs) reduced the macrophage inflammatory response. Knockdown of lamin A/C reduced macrophage inflammatory marker expression, while overexpression of lamin A/C significantly elevated inflammatory marker expression. We further found that suppression of lamin A/C by TNTs limited actin polymerization, thereby reducing the nuclear translocation of the actin-dependent transcriptional cofactor MRTF-A, which subsequently reduced the inflammatory response. In addition, emerin, which is a key link between lamin A/C and actin, was delocalized from the nucleus in response to mechanical stimulation by TNTs, resulting in reduced actin organization. Under inflammatory conditions, TNTs exerted favourable osteoimmunomodulatory effects on the osteogenic differentiation of mouse bone marrow-derived stem cells (mBMSCs) in vitro and osseointegration in vivo. This study shows and confirms for the first time that lamin A/C-mediated nuclear mechanotransduction controls macrophage inflammatory response, and this study provides a theoretical basis for the future design of immunomodulatory nanomorphologies on the surface of metallic bone implants."

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