Pregnancy Zone Protein Serves as a Prognostic Marker and Favors Immune Infiltration in Lung Adenocarcinoma

作者全名："Chen, Kehong; Zheng, Taihao; Chen, Cai; Liu, Liangzhong; Guo, Zhengjun; Peng, Yuan; Zhang, Xiaoyue; Yang, Zhenzhou"

作者地址："[Chen, Kehong; Zheng, Taihao; Chen, Cai; Liu, Liangzhong; Guo, Zhengjun; Peng, Yuan; Zhang, Xiaoyue; Yang, Zhenzhou] Chongqing Med Univ, Dept Canc Ctr, Affiliated Hosp 2, Chongqing 400010, Peoples R China"

通信作者："Zhang, XY; Yang, ZZ (通讯作者)，Chongqing Med Univ, Dept Canc Ctr, Affiliated Hosp 2, Chongqing 400010, Peoples R China."

来源：BIOMEDICINES

ESI学科分类：　

WOS号：WOS:001034912400001

JCR分区：Q1

影响因子：3.9

年份：2023

卷号：11

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：biomarker; lung adenocarcinoma; pregnancy zone protein; tumor infiltrating immune cells; macrophage; prognosis

摘要："Lung adenocarcinoma (LUAD) is a public enemy with a very high incidence and mortality rate, for which there is no specific detectable biomarker. Pregnancy zone protein (PZP) is an immune-related protein; however, the functions of PZP in LUAD are unclear. In this study, a series of bioinformatics methods, combined with immunohistochemistry (IHC), four-color multiplex fluorescence immunohistochemistry (mIHC), quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), were utilized to explore the prognostic value and potential role of PZP in LUAD. Our data revealed that PZP expression was markedly reduced in LUAD tissues, tightly correlated with clinical stage and could be an independent unfavorable prognostic factor. In addition, pathway analysis revealed that high expression of PZP in LUAD was mainly involved in immune-related molecules. Tumor immune infiltration analysis by CIBERSORT showed a significant correlation between PZP expression and several immune cell infiltrations, and IHC further confirmed a positive correlation with CD4+ T-cell infiltration and a negative correlation with CD68+ M0 macrophage infiltration. Furthermore, mIHC demonstrated that PZP expression gave rise to an increase in CD86+ M1 macrophages and a decrease in CD206+ M2 macrophages. Therefore, PZP can be used as a new biomarker for the prediction of prognosis and may be a promising immune-related molecular target for LUAD."

基金机构："Project of Chongqing Technology Innovation and Application Development [CSTC2021jscx-gksb-N0022]; National Natural Science Foundation of China [82273572, 82002448]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University [kryc-yq-2221]"

基金资助正文："This research was supported by the Project of Chongqing Technology Innovation and Application Development (No. CSTC2021jscx-gksb-N0022), National Natural Science Foundation of China (No. 82273572; No. 82002448) and the Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University (No. kryc-yq-2221)."