Downregulation of miR-182-5p by NFIB promotes NAD plus salvage synthesis in colorectal cancer by targeting NAMPT

作者全名："Zhou, Li; Liu, Hongtao; Chen, Zhiji; Chen, Siyuan; Lu, Junyu; Liu, Cao; Liao, Siqi; He, Song; Chen, Shu; Zhou, Zhihang"

作者地址："[Zhou, Li; Liu, Hongtao; Chen, Zhiji; Chen, Siyuan; Lu, Junyu; Liao, Siqi; He, Song; Zhou, Zhihang] Chongqing Med Univ, Affiliated Hosp 2, Dept Gastroenterol, Chongqing 400010, Peoples R China; [Liu, Cao] Gen Hosp Xinjiang Mil Command, Dept Emergency, Urumqi 830000, Peoples R China; [Chen, Shu] Chongqing Med Univ, Affiliated Hosp 2, Dept Hematol, Chongqing 400010, Peoples R China"

通信作者："Zhou, ZH (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Gastroenterol, Chongqing 400010, Peoples R China.; Chen, S (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Hematol, Chongqing 400010, Peoples R China."

来源：COMMUNICATIONS BIOLOGY

ESI学科分类：　

WOS号：WOS:001035550100002

JCR分区：Q1

影响因子：5.2

年份：2023

卷号：6

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Nuclear factor I B (NFIB) plays an important role in tumors. Our previous study found that NFIB can promote colorectal cancer (CRC) cell proliferation in acidic environments. However, its biological functions and the underlying mechanism in CRC are incompletely understood. Nicotinamide adenine dinucleotide (NAD+) effectively affects cancer cell proliferation. Nevertheless, the regulatory mechanism of NAD+ synthesis in cancer remains to be elucidated. Here we show NFIB promotes CRC proliferation in vitro and growth in vivo, and down-regulation of NFIB can reduce the level of NAD+. In addition, supplementation of NAD+ precursor NMN can recapture cell proliferation in CRC cells with NFIB knockdown. Mechanistically, we identified that NFIB promotes CRC cell proliferation by inhibiting miRNA-182-5p targeting and binding to NAMPT, the NAD+ salvage synthetic rate-limiting enzyme. Our results delineate a combination of high expression of NFIB and NAMPT predicted a clinical poorest prognosis. This work provides potential therapeutic targets for CRC treatment. Nuclear factor I B promotes colorectal cancer cell proliferation in vitro and growth in vivo by increasing intracellular NAD+ levels through downregulation of the NAMPT-targeting micro-RNA, miR-182-5p."

基金机构："National Natural Science Fund [81972285]; Senior Medical Talents Program of Chongqing for Young and Middle-aged, Chongqing Natural Science Foundation of China [CSTB2022NSCQ-MSX0312, CSTB2022NSCQ-MSX1038]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University [13-002-011, 13-004-009]; Young and Middle-aged Senior Medical Talents studio of Chongqing; Chongqing Medical Scientific Research Project (Joint Project of Chongqing Health Commission and Science and Technology Bureau) [2020FYYX103]"

基金资助正文："& nbsp;This work was supported by the National Natural Science Fund (No. 81972285), Senior Medical Talents Program of Chongqing for Young and Middle-aged, Chongqing Natural Science Foundation of China (CSTB2022NSCQ-MSX0312, CSTB2022NSCQ-MSX1038), Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University (13-002-011, 13-004-009), Young and Middle-aged Senior Medical Talents studio of Chongqing, and Chongqing Medical Scientific Research Project (Joint Project of Chongqing Health Commission and Science and Technology Bureau, 2020FYYX103)."