Neutrophil extracellular traps involved in the pathogenesis of IgA vasculitis: Confirmed in two IgAV rat models

作者全名："Chen, Xiu-Qi; Zou, Jia-Sen; Tu, Li; Yun, Xiang; Qin, Yuan-Han"

作者地址："[Chen, Xiu-Qi; Zou, Jia-Sen; Tu, Li; Yun, Xiang; Qin, Yuan-Han] Guangxi Med Univ, Affiliated Hosp 1, Dept Pediat, Nanning, Peoples R China; [Zou, Jia-Sen] Chongqing Med Univ, Affiliated Hosp 1, Chongqing, Peoples R China; [Tu, Li] Guangzhou Women & Childrens Med Ctr, Guangzhou, Peoples R China"

通信作者："Qin, YH (通讯作者)，Guangxi Med Univ, Affiliated Hosp 1, Dept Pediat, Nanning, Peoples R China."

来源：PLOS ONE

ESI学科分类：Multidisciplinary

WOS号：WOS:001038283600051

JCR分区：Q1

影响因子：2.9

年份：2023

卷号：18

期号：7

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："BackgroundNeutrophil extracellular traps (NETs) have been found to play a role in the development of autoimmune diseases. In the past two years, studies have demonstrated a significantly increase of NETs in skin tissues during the early stages of IgAV, indicating their involvement in disease activity among children with IgAV. However, the presence of NETs in IgAV animal models has not yet been reported. The objective of this study is to investigate whether NETs are involved in the pathogenesis of IgA vasculitis (IgAV) rats. MethodsTwenty-four SD rats were randomly divided into three groups: the ovalbumin group, the gliadin group, and the control group. The IgAV rat models were established administering Indian ink with ovalbumin (ovalbumin group) or gliadin (gliadin group) with Freund's complete adjuvant. The cell-free DNA (cf-DNA) was quantified by using dsDNA quantification kit, while the levels of Immunoglobulins, complement C3 and myeloperoxidase-DNA (MPO-DNA) in serum were tested using enzyme linked immunosorbent assay (ELISA). The IgA, complement C3 and NETs in tissues were detected through multiple immunofluorescences. ResultsBoth the ovalbumin group and gliadin group showed IgA and C3 deposition in various tissues, including the glomerular mesangial region, skin, and digestive tract, while the control group showed no such deposition. The levels of circulatory cf-DNA and MPO-DNA, which are components of NETs, were significantly elevated in both ovalbumin and gliadin groups compared with the control group. Furthermore, the presence of NETs were found in gastrointestinal and renal tissues of the ovalbumin and gliadin groups, but not in the control group. ConclusionsIgAV model rat can be established through the combination of ovalbumin and gliadin with Indian ink and Freund's complete adjuvant. This study provides the first confirmation that NETs are involved in the pathogenesis of IgAV rat."

基金机构："Innovation Project of Guangxi Graduate Education [YCBZ2021047]; Research Basic Ability Enhancement Project of Young and Middle-aged Teachers in Guangxi Universities [2021KY0096]; Excellent Medical Talents Training Program of the First Affiliated Hospital of Guangxi Medical University; Guangxi Medical High-level Backbone Talent ""139"" Plan [[G201901010]]; Key Laboratory of Children's Disease Research in Guangxi's Colleges and Universities, Education Department of Guangxi Zhuang Autonomous Region"

基金资助正文："This study was supported by Innovation Project of Guangxi Graduate Education (NO: YCBZ2021047); Research Basic Ability Enhancement Project of Young and Middle-aged Teachers in Guangxi Universities (NO: 2021KY0096); Excellent Medical Talents Training Program of the First Affiliated Hospital of Guangxi Medical University and Guangxi Medical High-level Backbone Talent ""139"" Plan (No. G201901010]) and the Key Laboratory of Children's Disease Research in Guangxi's Colleges and Universities, Education Department of Guangxi Zhuang Autonomous Region."