Bifidobacterium-derived membrane vesicles inhibit triple-negative breast cancer growth by inducing tumor cell apoptosis

作者全名："Jiang, Yongzhu; Wang, Lanxi; Yang, Bangya; Ma, Guanrong; Chen, Zhiqi; Ma, Jing; Chang, Xiulin; Fang, Liaoqiong; Wang, Zhibiao"

作者地址："[Jiang, Yongzhu; Wang, Lanxi; Yang, Bangya; Ma, Guanrong; Chen, Zhiqi; Ma, Jing; Chang, Xiulin; Fang, Liaoqiong; Wang, Zhibiao] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Fang, Liaoqiong] Natl Engn Res Ctr Ultrasound Med, Chongqing 401121, Peoples R China; [Wang, Zhibiao] Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China"

通信作者："Fang, LQ; Wang, ZB (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China.; Fang, LQ (通讯作者)，Natl Engn Res Ctr Ultrasound Med, Chongqing 401121, Peoples R China.; Wang, ZB (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China."

来源：MOLECULAR BIOLOGY REPORTS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:001038585000003

JCR分区：Q3

影响因子：2.6

年份：2023

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期号：　

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结束页：　

文献类型：Article; Early Access

关键词：Bifidobacterium; Membrane vesicles; Triple-negative breast cancer; Apoptosis

摘要："Background Bacterial outer membrane vesicles have gained increasing attention for its antitumor effect and application in drug delivery. However, the bacterial membrane vesicles (MVs) that are secreted by Gram-positive bacteria are rarely mentioned. Bifidobacterium has a certain anti-tumor effect, but there is a certain risk when injected into human body. Here we investigated the potential of Bifidobacterium-derived membrane vesicles (B-MVs) as therapeutic agents to treat triple-negative breast cancer.Methods and results Firstly, we discovered that Bifidobacterium can produce B-MVs and isolated them. In vivo, we found that B-MVs can inhibit tumor growth in mice and the mice were in good state. H & E staining displayed extensive apoptotic cells in tumor tissues. Western blotting and immunohistochemistry showed that B-MVs increased the expression of Bax, while decreased the expression of Bcl-2. These results suggested that B-MVs may induce apoptosis of tumor cells in vivo. Furthermore, to further confirm this phenomenon, we conducted experiments in vitro. Hoechst 33,258 staining assay, flow cytometry and western blotting also demonstrated B-MVs promoted cell apoptosis in vitro.Conclusions We speculate B-MVs may inhibit tumor growth by inducing tumor cell apoptosis in triple-negative breast cancer, which provided a new direction in the treatment of TNBC.{GRAPHIACAL ABSTRACT}"

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