A three-site recognition cytosensor based on multi-active AuIrPt polyhedral nanozymes for detection of CTCs

作者全名："Li, Baiying; Shen, Huawei; Liu, Qian; Liu, Xin; Cai, Juan; Zhang, Li; Wu, Di; Xie, Yaxing; Xie, Guoming; Feng, Wenli"

作者地址："[Li, Baiying; Liu, Xin; Wu, Di; Xie, Yaxing; Xie, Guoming; Feng, Wenli] Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Shen, Huawei] Chongqing Hosp Tradit Chinese Med, Clin Lab Med Ctr, Chongqing 400021, Peoples R China; [Liu, Qian] Chongqing Med Univ, Dept Nucl Med, Hosp 2, Chongqing 400010, Peoples R China; [Cai, Juan] Army Med Univ, Southwest Hosp, Dept Clin Lab Med, Chongqing 400038, Peoples R China; [Zhang, Li] Chongqing Med Univ, Coll Basic Med, Lab Forens Med & Biomed Informat, Chongqing 400016, Peoples R China; [Xie, Guoming] Chongqing Med Univ, Minist Educ, Dept Lab Med, Key Lab Med Diagnost,Chongqing Med Lab Microfluid, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Xie, Guoming] Chongqing Med Univ, SPRi Engn Res Ctr, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China; [Feng, Wenli] Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost, Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China"

通信作者："Xie, GM (通讯作者)，Chongqing Med Univ, Minist Educ, Dept Lab Med, Key Lab Med Diagnost,Chongqing Med Lab Microfluid, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China.; Xie, GM (通讯作者)，Chongqing Med Univ, SPRi Engn Res Ctr, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China.; Feng, WL (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Med Diagnost, Minist Educ, 1 Yi Xue Yuan Rd, Chongqing 400016, Peoples R China."

来源：SENSORS AND ACTUATORS B-CHEMICAL

ESI学科分类：CHEMISTRY

WOS号：WOS:000978505400001

JCR分区：Q1

影响因子：8.4

年份：2023

卷号：386

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Circulating tumor cells; Three -site recognition strategy; Multi -active AuIrPt polyhedral nanozymes; Electrochemical cytosensor

摘要："We developed a novel electrochemical cytosensor based on AuIrPt nanozymes and three-site recognition strategy for the detection of specific CTCs. Using pluronic F127 as the organic framework, new trimetallic AuIrPt nanozymes with exceptional multiple enzyme-like activities were synthesized, which could mimic the activities of three enzymes, SOD, POD, and CAT. Nanozymes linked with EpCAM (Epithelial cell adhesion molecule) aptamers served as signal tags to enhance sensitivity of the cytosensor, and anti-MUC1 (Mucin 1) and anti-EGFR (Epidermal growth factor receptor) antibodies for isolation and capture of the target CTCs, respectively. The AuIrPt nanozymes could approach the sensing interface and generate obvious currents through their exceptional catalytic activity only when all three proteins (MUC1, EpCAM, and EGFR) were simultaneously expressed on the cell membrane. Benefiting from the aforementioned advantages, our cytosensor could accurately distinguish between target cells and other cancer cells with a wide concentration range from 5 to 1 x 106 cells mL-1 and a low detection limit of 2 cells mL-1. Notably, the biosensor could assay target CTCs in complex whole blood matrixes, validating its resistance to interference and utility. By combining catalytic nanomaterials and three-site recognition strategy, a sensitive, accurate, and stable novel sensing platform has been developed, promising to facilitate the application of CTCs in early cancer screening."

基金机构：National Natural Science Foundation of China [81902171]; Top Talent Project of Chongqing Medical University [BJRC202124]

基金资助正文：This work was financially supported by the National Natural Science Foundation of China (No. 81902171) and the Top Talent Project of Chongqing Medical University (BJRC202124) .