A low-cost paper-based blood urea nitrogen optical biosensor for renal surveillance in fingertip blood

作者全名："Cheng, Jie; Fu, Yusheng; Guo, Jiuchuan; Guo, Jinhong"

作者地址："[Guo, Jinhong] Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai 200240, Peoples R China; [Cheng, Jie; Fu, Yusheng; Guo, Jinhong] Univ Elect Sci & Technol China, Sch Informat & Commun Engn, Chengdu 611731, Peoples R China; [Guo, Jiuchuan] Univ Elect Sci & Technol China, Sch Automation Engn, Chengdu 611731, Peoples R China; [Guo, Jinhong] Chongqing Med Univ, Coll Lab Med, MOE Key Lab Lab Med Diagnost, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China"

通信作者："Guo, JH (通讯作者)，Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai 200240, Peoples R China.; Guo, JC (通讯作者)，Univ Elect Sci & Technol China, Sch Automation Engn, Chengdu 611731, Peoples R China."

来源：SENSORS AND ACTUATORS B-CHEMICAL

ESI学科分类：CHEMISTRY

WOS号：WOS:000978606900001

JCR分区：Q1

影响因子：8.4

年份：2023

卷号：387

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：POCT; BUN bioassay; Optical biosensor; Photochemistry; Colorimetric; Smartphone-enabled biosensor

摘要："In this study, we developed a low-cost, handheld optical biosensor for measuring blood urea nitrogen (BUN) levels in fingertip blood. This biosensor can connect to a cloud-enabled smartphone for remote monitoring of renal diseases. The biosensor features a simple, stackable and multilayer paper-based strip design, which not only achieves a linear optical fast response to BUN concentration but also reduces the complexity of the optical reader. Excellent detection performance was obtained via a unique photoelectric detection algorithm. The proportional function for calculating the analyte concentration was established using blood samples with different BUN concentrations, resulting in a wide detection range of 2.46-38.14 mM and a low detection limit of 0.03 mM. Clinical sample testing demonstrated the biosensor's high reliability, showing strong agreement with the bulky biochemistry analyzer (gold standard), with a correlation coefficient R2 = 0.9635. It is also capable of connecting to a cloud-enabled smartphone via Bluetooth to transmit test data, which has an easy-to-use interface, thus enabling proactive health management or remote diagnosis. We believe that the proposed BUN biosensor can be a big boost to the daily monitoring of renal diseases and has promising prospects in contributing to the development of the IoMT."

基金机构：National Natural Sci- ence Foundation of China [62122017]

基金资助正文：The authors thank the financial support from National Natural Sci- ence Foundation of China (62122017) .