Circular RNA RSU1 promotes retinal vascular dysfunction by regulating miR-345-3p/TAZ

作者全名："Zhang, Yiting; Hu, Jianping; Qu, Xiaoying; Hu, Ke"

作者地址："[Zhang, Yiting; Hu, Ke] Chongqing Med Univ, Affiliated Hosp 1, Chongqing Eye Inst, Chongqing Key Lab Ophthalmol, Chongqing, Peoples R China; [Zhang, Yiting; Hu, Ke] Natl Clin Res Ctr Ocular Dis, Chongqing Branch, Chongqing, Peoples R China; [Hu, Jianping] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Dept Ophthalmol, Shanghai, Peoples R China; [Hu, Jianping] Shanghai Key Lab Orbital Dis & Ocular Oncol, Shanghai, Peoples R China; [Qu, Xiaoying] Chongqing Med Univ, Hlth Management Ctr, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Hu, K (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Chongqing Eye Inst, Chongqing Key Lab Ophthalmol, Chongqing, Peoples R China.; Hu, K (通讯作者)，Natl Clin Res Ctr Ocular Dis, Chongqing Branch, Chongqing, Peoples R China."

来源：COMMUNICATIONS BIOLOGY

ESI学科分类：　

WOS号：WOS:001024877700002

JCR分区：Q1

影响因子：5.9

年份：2023

卷号：6

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："The circular RNA circRSU1 is overexpressed in diabetic retinopathy patients and circRSU1 sponges miR-345-3p, increasing TAZ levels, whose downregulation can reverse vascular dysfunction. Diabetic mellitus-induced diabetic retinopathy is a significant cause of visual impairment and blindness in adults. Circular RNAs (circRNAs) have been shown to play initial roles in vascular progression. However, the mechanism underlying diabetes mellitus-induced vascular complications remains largely unknown. In circRNA chip experiments, circRSU1 was found to be generally overexpressed in diabetic retinopathy patients. Human retina endothelial cells were stably transfected with lentiviruses carrying a circRSU1 interference plasmid. CircRSU1 downregulation alleviated diabetes mellitus induced retina vascular dysfunction, resulting in decreased vascular endothelial growth factor levels, inflammatory responses and oxidative stress. Mechanistically, we showed that elevated circRSU1 expression upregulated the TAZ levels by sponging miR-345-3p. Downregulation of TAZ reversed the vascular dysfunction that was caused by increased circRSU1 expression under hyperglycaemic conditions. In conclusion, overexpression of circRSU1 promotes vascular dysfunction by sponging miR-345-3p to increase the TAZ levels under diabetic conditions. We provide evidence that circRSU1 is a potential therapeutic target for treating diabetes mellitus-induced vascular dysfunction."

基金机构：National Natural Science Foundation of China [81900887]

基金资助正文：AcknowledgementsWe are grateful to all participates for their contributions in this study. This study was supported by the National Natural Science Foundation of China (81900887).