Engineering hetero-structural iron nanozyme decorated liposome with a self-cascade catalysis performance

作者全名："Wang, Teng; Wu, Qing; Wang, Zhenyu; Hu, Xi; Mao, Xiang"

作者地址："[Wang, Teng; Wu, Qing; Wang, Zhenyu; Hu, Xi; Mao, Xiang] Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China; [Wang, Teng; Wu, Qing; Wang, Zhenyu; Hu, Xi; Mao, Xiang] Chongqing Med Univ, Coll Biomed Engn, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China"

通信作者："Mao, X (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, State Key Lab Ultrasound Med & Engn, Chongqing 400016, Peoples R China.; Mao, X (通讯作者)，Chongqing Med Univ, Coll Biomed Engn, Chongqing Key Lab Biomed Engn, Chongqing 400016, Peoples R China."

来源：BIOMATERIALS SCIENCE

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:001034686100001

JCR分区：Q2

影响因子：6.6

年份：2023

卷号：　

期号：　

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文献类型：Article; Early Access

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摘要："Metal-based enzyme mimics are considered as acceptable agents in fabricating heterogeneous biocomposites through valency integrations because of their biomedical or biological properties. As the basic substitute, it delights us to utilize Fe3O4 nanoparticles (NPs) as metallic enzymes and overcome the limitation of peroxide-like enzymatic activity in physiological conditions. In this work, we present the fabrication of a soy phosphatidylcholine/Fe3O4@Ag/GOx (SFAG) biocomposite as a cascade enzyme, which exhibits a peroxidase-like property in kinetic processes, as shown from an analysis of the glucose detection processes. We also explored the mechanism of an ultrasound & microfluidic approach for the synthesis of SFAG. The resultant SFAG implies a characteristic absorption peak (652 nm), size (55 & mu;m), and surface charge (-32.93 & PLUSMN; 2.58 mV). This is utilized to confirm the peroxidase-like activity by catalyzing 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) in the presence of H2O2 under physiological conditions. But also, SFAG conveys a positive effect on the peroxidase-like activity at pH = 5.8, 7.4, and 8.0. The Michaelis-Menten parameters (K-m) and the V-max values of H2O2 are 1.914 mM and 1.429 x 10(-7) M s(-1), which further confirms the catalytic performances of the SFAG structure. The established platform was also used successfully for the determination of glucose in PBS and diluted synthetic blood with excellent sensitivity and stability. The relative selection and sensitivity show that the SFAG structure has a great possibility as a cascade metallic enzyme in chemokinetic works."

基金机构：Chongqing Municipal Natural Science Foundation [CSTB2022NSCQ-MSX0113]; Scientific Foundation Project of Chongqing Education Commission [KJQN201900436]

基金资助正文：This work was supported by the Chongqing Municipal Natural Science Foundation (CSTB2022NSCQ-MSX0113) and the Scientific Foundation Project of Chongqing Education Commission (KJQN201900436).