T-lymphocytes from focused ultrasound ablation subsequently mediate cellular antitumor immunity after adoptive cell transfer immunotherapy

作者全名："Ran, Li-Feng; Xie, Xun-Peng; Xia, Ji-Zhu; Xie, Fang-Lin; Fan, Yan-Min; Wu, Feng"

作者地址："[Ran, Li-Feng] Chongqing Med Univ, Affiliated Hosp 2, Clin HIFU Ctr Tumor Therapy, Chongqing, Peoples R China; [Ran, Li-Feng; Xie, Xun-Peng; Xia, Ji-Zhu; Xie, Fang-Lin; Fan, Yan-Min; Wu, Feng] Chongqing Med Univ, Inst Ultrason Engn Med, Chongqing, Peoples R China; [Xie, Xun-Peng] Nantong Univ, Nantong Peoples Hosp 3, Dept Oncol, Nantong, Jiangsu, Peoples R China; [Wu, Feng] Univ Oxford, Nuffield Dept Surg Sci, Oxford, England"

通信作者："Wu, F (通讯作者)，Chongqing Med Univ, Inst Ultrason Engn Med, Chongqing, Peoples R China.; Wu, F (通讯作者)，Univ Oxford, Nuffield Dept Surg Sci, Oxford, England."

来源：FRONTIERS IN IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:001043441300001

JCR分区：Q1

影响因子：7.3

年份：2023

卷号：14

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：high intensity focused ultrasound; ablation; liver cancer; immunomodulation; adoptive cell transfer; immunotherapy; apoptosis; cytotoxic T lymphocyte

摘要："BackgroundOur previous studies found that high-intensity focused ultrasound (HIFU) stimulated tumor-specific T cells in a mouse H-22 tumor model, and adoptive transfer of the T cells from HIFU-treated mice could subsequently elicit stronger inhibition on the growth and progression of the implanted tumors. The aim of this study was to investigate the mechanism of T cells from focused ultrasound ablation in HIFU-mediated immunomodulation. MethodsSixty H-22 tumor-bearing mice were treated by either HIFU or sham-HIFU, and 30 naive syngeneic mice served as controls. All mice were euthanized on day 14 after HIFU and splenic T cell suspensions were obtained in each group. Using an adoptive cell transfer model, a total of 1 x 10(6) T cells from HIFU treated-mice were intravenously injected into each syngeneic H-22 tumor-bearing mouse twice on day 3 and 4, followed by the sacrifice for immunological assessments at 14 days after the adoptive transfer. ResultsT cells from HIFU-treated mice could significantly enhance the cytotoxicity of CTLs (p < 0.001), with a significant increase of TNF-& alpha; (p < 0.001) and IFN-& gamma; secretion (p < 0.001). Compared to control and sham-HIFU groups, the number of Fas ligand(+) and perforin(+) tumor-infiltrating lymphocytes (TILs) and apoptotic H-22 tumor cells were significantly higher (p < 0.001) in the HIFU group. There were linear correlations between apoptotic tumor cells and Fas ligand(+) TILs (r = 0.9145, p < 0.001) and perforin(+) TILs (r = 0.9619, p < 0.001). ConclusionT cells from HIFU-treated mice can subsequently mediate cellular antitumor immunity, which may play an important role in the HIFU-based immunomodulation."

基金机构："Ministry of Science and Technology of China, 973 Program [2011CB707900]"

基金资助正文："Funding This study was funded by Ministry of Science and Technology of China, 973 Program, grant number 2011CB707900."